电针夹脊穴的抗炎镇痛和免疫调节作用(英文)  

Effect of electropumcture at Jiaji acupoint on anti-inflammation,analgesia and immunoloregulation

作　　者：吕玉玲[1] 赵鲁明[2] 陈洪沛[1] 房繄恭[2] 吴富东[2] 

机构地区：[1]成都中医药大学针推学院 [2]山东中医药大学针推学院,山东省济南市250014

出　　处：《中国临床康复》2006年第19期171-173,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助课题(30371801)~~

摘　　要：背景:临床观察证实,针灸具有确切的抗炎和免疫调节的作用,这是针灸防治各类免疫失调和急慢性炎症的基础。目的:观察电针夹脊(对佐剂性关节炎大鼠的抗炎镇痛作用及对其T细胞亚群的影响。设计:完全随机分组设计,对照实验。单位:山东中医药大学针灸基础实验室。材料:选用纯种Wistar大鼠30只。适应性饲养5d后,随机将大鼠分为3组:正常对照组,模型组,电针夹脊(组,每组10只。方法:①实验于2005-01/2005-05在山东中医药大学针灸基础实验室完成。模型组和电针夹脊(组用弗氏完全佐剂造成佐剂性关节炎模型。于造模当天,电针夹脊(组针刺双侧第3,5腰椎夹脊(,用28号0.5寸(约1.65cm)不锈钢毫针在大鼠第3,5腰椎棘突下旁开约0.3cm直刺进针,接G6805-2A型多功能电针治疗仪给予疏密波(频率约4Hz,密波频率约60Hz),强度约为1mA,30min/次,1次/d,连续治疗7d。正常对照组及模型组以相同的方式捆绑固定(用粗布绳将大鼠捆绑固定于大鼠固定器)7d。②于造模前,造模后1,7d进行痛阈测定:采用热痛刺激仪的强光照射大鼠足垫部,将大鼠的缩爪潜伏期作为痛阈值。③造模前,造模后1,7d采用鼠足容积测定器测定大鼠右后爪足容积(容积排水法),计算肿胀率(%)=(造模后鼠足容积-造模前鼠足容积)/造模前鼠足容积×100%。④于造模后8d,采用FACSCalibur流式细胞仪检测血清CD4+,CD8+细胞表达率并计算CD4+/CD8+。⑤多个样本均数比较采用单因素方差分析和t检验。主要观察指标:电针夹脊(对佐剂性关节炎大鼠痛阈、足肿胀率及血清T细胞亚群的影响。结果:大鼠30只均进入结果分析。①造模后1d,模型组、电针夹脊(组大鼠右后足足容积明显高于正常对照组和造模前(P<0.01);肿胀率明显高于正常对照组(P<0.01)。造模后7d,模型组右后足容积及足爪肿胀率明显高于正常对照组(P<0.01),足容积明显高于造模前(P<0.01);电针�BACKGROUND： Clinical observation has been proved that acupuncture has effect on anti-inflammation and immunoloregulation, which is the basis for preventing and curing immune disturbance and active chronic inflammation. OBJECTIVE： To observe the effect of electropumcture on antinflammation, analgesia and subgroup of T cells of adjuvant-arthrosis rats at Jiaji acupoint. DESIGN： Completely randomized grouping and controlled study. SETTING： Basic Laboratory of Acupuncture and Moxibustion, Shandong University of Traditional Chinese Medicine. MATERIALS： A total of 30 Wistar rats were selected in this study. Five days after feeding, all rats were randomly divided into 3 groups： normal control group, model group and electropumcture group with 10 in each group. METHODS： ①The experiment was completed at Basic Laboratory of Acupuncture and Moxibustion of Shandong University of Traditional Chinese Medicine from January to May 2005, Freund＇s complete adjuvant （FCA） was used to establish adjuvant-arthrosis models in model group and electropumcture group. On the modeling day, the 3^rd and the 5^th Jiaqi acupoints of bilateral lumbar vertebrae of rats in electropumcture group were acupunctured with 28-sized stainless-steel milli-needle of 0.5 inch. The needle was stimulated 0.3 cm from the 3^rd and the 5^th spinous process of lumbar vertebra which was connected to G6805-2A multiple functional eleetropumeture meter for sparse-tight waves （frequency of sparse wave： 4 Hz, frequency of tight wave： 60 Hz, intensity： 1 mA） 30 minutes each time, once a day for 7 days. Rats in normal group and model group were fixed with the same way for 7 days. （Rats were fixed with cloth-rope at fixing apparatus.） ② Pain threshold was measured before modelling, 1 and 7 days after modelling. Font pad was exposured with strong light by hot-pain stimulation meter, and paw withdrawal latency was regarded as pain threshold. ③Right hindfoot bulk of rats was assayed with foot bulk determinator （bulk draining

关 键 词：关节炎 佐剂性/针灸疗法 电针 疾病模型 动物 镇痛 消炎 T淋巴细胞亚群 

分 类 号：R245[医药卫生—针灸推拿学]