罗勒(Ocimumbasilicum)悬浮细胞系的建立与保持  

Establishment and Maintenance of Cell Suspensions of Ocimum basilieum

作　　者：蔡汉权[1] 赖钟雄[2] 林珊珊[2] 林庆良[2] 

机构地区：[1]广东韩山师范学院,广东潮州521041 [2]福建农林大学园艺植物生物研究所,福建农林大学园艺学院福州350002

出　　处：《热带作物学报》2006年第1期44-48,共5页Chinese Journal of Tropical Crops

基　　金：农业部948计划资助项目(991029)资助

摘　　要：通过罗勒茎尖培养获得疏松型(CA)、泥状型(CB)和绿色块状型(CC)愈伤组织;以这3种愈伤组织为起始材料,研究了建立罗勒悬浮细胞系的方法、影响因素及继代保持的方法。结果表明:疏松型、泥状型愈伤组织均能建立悬浮细胞系;以泥状型愈伤组织为起始材料,建立悬浮细胞系时间仅需7￣8d。The shoot tip of Ocimum basilieum was induced and cultured to obtain three types of calli, CA, CB and CC. The factors affecting establishment of suspensions extracted from these calli, and the methods of suspension maintenance were studied. The results showed that both the CA and CB calli could be used as initial materials for establishing suspensions, and that it took only 7～8 days to establish cell suspensions from the CB callus.

关 键 词：罗勒 愈伤组织 悬浮细胞培养 

分 类 号：Q943.1[生物学—植物学] S573.9[农业科学—作物学]