白介素-4和白介素-13促进人肺成纤维细胞ADAM33基因的表达  

Interleukin-4(IL-4) and IL-13 enhance ADAM33 gene expression in human lung fibroblasts

作　　者：揭志军[1] 金美玲[1] 蔡映云[1] 袁正宏[2] 胡芸文[2] 徐杨[2] 

机构地区：[1]复旦大学附属中山医院呼吸病研究所 [2]复旦大学上海医学院教育部医学分子病毒学重点实验室,上海200032

出　　处：《复旦学报（医学版）》2006年第3期295-300,共6页Fudan University Journal of Medical Sciences

基　　金：上海市卫生局科技发展基金(034094)资助

摘　　要：目的探讨白介素-4(IL-4)和白介素-13(IL-13)对人肺成纤维细胞ADAM33(a disintegrin and a metal-loproteinase33,ADAM33)mRNA表达的影响。方法以不同浓度的IL-4或/和IL-13刺激培养的人肺成纤维细胞(MRC-5),然后用实时定量反转录多聚酶链反应(real-time RT-PCR)法测定ADAM33mRNA表达的变化。结果人肺成纤维细胞上存在着ADAM33mRNA的表达,当分别以10ng/mL的IL-4或IL-13刺激时,AD-AM33mRNA的表达增加呈现时间依赖性,至24h达到高峰。随着IL-4和IL-13刺激浓度的增加,ADAM33mRNA的表达也明显增加:以100ng/mL的IL-4和100ng/mL的IL-13刺激时,ADAM33mRNA的表达较未刺激细胞分别增加了(9.49±2.83)倍和(14.21±3.35)倍(P<0.01);而以10ng/mL的IL-4和10ng/mL的IL-13联合刺激时,ADAM33mRNA的表达较未刺激细胞增加了(15.06±4.57)倍(P<0.01)。结论IL-4和IL-13能明显促进肺成纤维细胞ADAM33mRNA的表达。Purpose To study the effect of IL-4 and IL-13 in ADAM33 gene expression on lung fibroblast. Methods Human fetal lung fibroblasts （MRC-5） were cultured and stimulated with IL-4 or/and IL-13. The expression of ADAM33 mRNA was evaluated by real-time reverse transcriptasepolymerase chain reaction （real-time RT-PCR）. Results ADAM33 mRNA was expressed in lung fibroblasts. IL-4 and IL-13 enhanced the expression of ADAM33 mRNA in a time- and dose-dependent manner. When stimulated with 10 ng/mL IL-4 or 10 ng/mL IL-13 for 24 h, the expression of AD- AM33 mRNA significantly increased to crest. When stimulated with 100 ng/mL IL-4 or 100 ng/mL IL-13, the expression of ADAM33 mRNA significantly increased by 9.49 ± 2.83 or 14.21 ± 3.35 times compared with unstimulated cells （P〈0.01）. When stimulated with 10 ng/mL IL-4 and 10 ng/mL IL- 13, the expression of ADAM33 mRNA significantly increased by 15.06 ± 4.57 times （P〈0.01）. Conclusions These results suggest that ADAM33 mRNA is expressed on lung fibroblasts and IL-4 and IL-13 can enhance ADAM33 expression.

关 键 词：分裂素区和金属蛋白酶区基因 哮喘 成纤维细胞 气道重塑 

分 类 号：R562.25[医药卫生—呼吸系统]