红藤饮片提取物抑菌活性与次生代谢产物含量的相关性  

Correlation of anti-bacterial activity with secondary metabolites content in Sargentodoxa Cuneata tables

作　　者：李钧敏[1] 金则新[1] 陈彤[1] 顾奇萍[1] 

机构地区：[1]台州学院生态研究所,浙江临海317000

出　　处：《浙江大学学报（医学版）》2006年第3期273-280,共8页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(39870160);浙江省高校中青年教师资助计划项目(2003年)

摘　　要：目的:了解3个不同产地、2个不同批次的红藤饮片6种次生代谢产物含量、抑菌活性及两者的相关性。方法:利用分光光度法对红藤饮片提取物的6种次生代谢产物含量进行分析,以金黄色葡萄球菌和枯草芽孢杆菌为实验菌株,用纸片琼脂扩散法测定抑菌圈大小;以平板二倍稀释法统计最小抑菌浓度(M IC);以试管二倍稀释法统计最小杀菌浓度(M BC)。结果:红藤药液对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性以安徽产地第一批次最高,抑菌圈大小、M IC、M BC分别为(9.67±0.29)mm、12.5 m g/m l、31.25 m g/m l和(10.17±0.58)mm、12.5 m g/m l、31.25 m g/m l,江苏产地第二批次最低,抑菌圈大小、M IC、M BC分别为(7.67±0.29)mm、25 m g/m l、125 m g/m l和(8.17±0.29)mm、25 m g/m l、125 m g/m l。红藤饮片提取物对金黄色葡萄球菌的抑菌活性在不同产地和不同批次之间均有显著性差异(P<0.01);对枯草芽孢杆菌的抑菌活性在不同产地亦有显著性差异(P<0.01),但不同批次之间无显著性差异(P>0.05)。红藤药液的6种次生代谢产物总量以安徽产地第一批次最高(3.61%),江苏产地第二批次最低(1.90%)。红藤提取物的总生物碱和游离蒽醌在不同产地之间有显著性差异(P<0.01),而在不同批次之间无显著性差异(P>0.05);总绿原酸、黄酮类化合物、总鞣质和总皂苷在不同产地和不同批次之间均有显著性差异(P<0.01)。影响红藤饮片对2种细菌的抑菌活性的主要次生代谢产物可能为总皂甙、总鞣质、游离蒽醌及总绿原酸。结论:不同红藤饮片的次代谢产物的含量及抑菌活性以安徽和浙江产地较高,江苏产地较低;红藤的抑菌活性与饮片中总皂甙、总鞣质、游离蒽醌及总绿原酸的含量密切相关。Objective： To explore the correlation between the content of the secondary metabolites and the anti-bacterial activity of Sargentodoxa cuneata tables. Methods： The content of secondary metabolies was determined by spectrometers. Staphylococcus aureus and Bacillus subtilis were used as the experimental strains. The minimal inhibition concentration （MIC） was calculated using agar plate dilution method on Kirby-Bauer disk. The minimal baeterialeidal concentration （MBC） was calculated using tube dilution method. Two-way analysis and Turkey＇s test was employed in the statistical analysis. Results： The anti-bacterial activities on Staphylococcus aureus and Bacillus subtilis of the first batch of S. cuneata tables produced in Anhui was the highest [the diameter of anti-bacterial zone,MIC,MBC was （9.67±0.29） mm, 12.5 mg/ ml,31.25 mg/ml and （10.17±0.58） mm,12.5 mg/ml,31.25 mg/ml,respectively]. Those of the second batch of S. cuneata tables produced in Jiangsu was the lowest [the diameter of anti- bacterial zone,MIC,MBC was （7.67±0.29）mm,25 mg/ml,125 mg/ml and （8.17±0.29）mm,25 mg/ml, 125 mg/ml ,respectively Staphylococcus aureus between There was significant difference in the antibacterial activity on fferent producing areas of S. cuneata tables and batches （P〈 0. 01） , while there was also significant difference in the antibacterial activity on Bacillus subtilis between different producing areas （P〈0.01） and no significant difference between different batches （P〉0.05）. The contents of 6 secondary metabolites of S. cuneata tables produced in different areas and in different batches were different. The total content of 6 secondary metabolites of the first batch of S. cuneata tables produced in Anhui was the highest with mean 3.61%. Those of the second batch produced in Jiangsu were the lowest with mean 1.90%. There was significant difference in the content of total alkaloid and dissociated emodin between different producing areas （P~ 0.01） and no difference between

关 键 词：大血藤/分离和提纯 大血藤/药理学 大血藤/分析 葡萄球菌 金黄色/药物作用 芽孢杆菌 枯草/药物作用 抗菌药/药理学 

分 类 号：R285[医药卫生—中药学]