Preprocessing of Tandem Mass Spectrometric Data Based on Decision Tree Classification  

作　　者：Jing-Fen Zhang Si-Min He Jin-Jin Cai Xing-Jun Cao Rui-Xiang Sun Yan Fu Rong Zeng Wen Gao 

机构地区：[1]Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China [2]Graduate School of Chinese Academy of Sciences, Beijing 100080, China [3]Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

出　　处：《Genomics, Proteomics & Bioinformatics》2005年第4期231-237,共7页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by the National Basic Research Program(973 Program)of China(No.2002CB713807);the National Key Technologies R&D Program of China(No.2004BA711A21)

摘　　要：In this study, we present a preprocessing method for quadrupole time-of-flight （Q-TOF） tandem mass spectra to increase the accuracy of database searching for peptide （protein） identification. Based on the natural isotopic information inherent in tandem mass spectra, we construct a decision tree after feature selection to classify the noise and ion peaks in tandem spectra. Furthermore, we recognize overlapping peaks to find the monoisotopic masses of ions for the following identification process. The experimental results show that this preprocessing method increases the search speed and the reliability of peptide identification.In this study, we present a preprocessing method for quadrupole time-of-flight （Q-TOF） tandem mass spectra to increase the accuracy of database searching for peptide （protein） identification. Based on the natural isotopic information inherent in tandem mass spectra, we construct a decision tree after feature selection to classify the noise and ion peaks in tandem spectra. Furthermore, we recognize overlapping peaks to find the monoisotopic masses of ions for the following identification process. The experimental results show that this preprocessing method increases the search speed and the reliability of peptide identification.

关 键 词：peptide identification Q-TOF tandem mass spectra feature selection decision tree 

分 类 号：Q5[生物学—生物化学]