中药复方对糖尿病大鼠肾脏TGF—β_1 mRNA表达的影响  

Effect of Compound Formula of Chinese Herbal on Kidney TGF-β_1 mRNA Expression of Biabetic Rats

作　　者：姚祈[1] 张兰[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《辽宁中医杂志》2006年第5期631-632,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：辽宁省教育厅计划项目(202153331)

摘　　要：目的:观察健脾益肾活血化瘀法组成的中药复方对早期糖尿病肾病(DN)大鼠肾皮质转化生长因子β1(TGFβ1)的影响,探讨其作用机理。方法:实验采用链脲佐菌素(STZ)按53mg/kg剂量腹腔一次性注射建立糖尿病肾病大鼠模型,造模成功后随机分为中药组(中药复方)、西药组(瑞泰)、模型组和正常组。灌胃8周后RT—PCR法检测大鼠肾皮质TGFβ1mRNA表达。结果:各治疗组与模型组比较均明显降低糖尿病大鼠肾皮质TGFβ1mRNA表达(P<0.01),且中药组优于西药组(P<0.01)。结论:中药复方通过降低糖尿病大鼠肾皮质TGFβ1mRNA的表达,从而起到保护肾脏,延缓肾脏病理损害,抑制糖尿病肾病形成的作用。Objective：To observe the effects of compound formula of Chinese herbal benefiting spleen and kidney and invigorating blood on kidney cortex transforming growth factorβ1 （ TGFβ1 ） expression, and probe its mechanism in rats with early diabetic nephropathy（DN）. Methods：DN is induced by streptozocin（STZ） in rats according to 53mg/kg i. p. Then randomly assign to traditional Chinese medicine group （compound formula）, western medicine group （ Ruitai）, model group and normal group. Eight weeks later, measure the expression of the rats kidney cortex TGFβ1 with RT - PCR. Results：Compared with the model group, TGFβ1 （P 〈0.01 ） is obviously lowerer in the treating group. And the traditional Chinese medicine group is superior to the Western medicine group （P 〈 0.01 ）. Conclusion： Compound formula of Chinese herbal can protect kidney, delay pathology damage to kidney, and hold back from DN by lowering expression level of TGFβ1.

关 键 词：中药复方 糖尿病大鼠 转化生长因子Β1 

分 类 号：R285.5[医药卫生—中药学] R587.5[医药卫生—中医学]