ERβ相互作用蛋白PSMC5的分离及其在ERβ信号途径中的作用  

Isolation of an ERβ-interacting Protein PSMC5 and Its Role in ERβ Signaling

作　　者：王晓辉[1] 张浩[1] 周蕾[1] 丁丽华[1] 韩聚强[1] 宁康[1] 孙琰[1] 袁斌[1] 刘辉[1] 常庆[1] 李杰之[1] 叶棋浓[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850

出　　处：《中国生物化学与分子生物学报》2006年第5期384-389,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.30428012;No.30470378和No.30530320)~~

摘　　要：雌激素受体β(ERβ)在乳腺癌发生发展中起着重要的作用,寻找与ERβ相互作用的共调节因子对阐明ER信号通路具有重要价值.应用酵母双杂交技术,以ERβ的AF2结构域为诱饵蛋白从人乳腺文库中筛选出了与之相互作用的蛋白26S蛋白酶的亚单位ATPase5(PSMC5).GST沉淀实验表明,PSMC5在体外特异地与ERβ相结合.转录活性实验表明,PSMC5以激素依赖的方式降低ERβ转录活性.上述结果提示,PSMC5可能通过影响ERβ信号途径在乳腺癌发生发展中起着重要的作用.Estrogen receptor β （ERβ） palys an important role in the development and progression of breast cancer. It is of great significance to discover ERβ-interacting coregulators. Using a yeast two-hybrid system with the AF2 domain of EPβ as bait, PSMC5, the proteasome 26S subunit ATPase 5, was isolated from a human mammary library to interact with ERβ. GST pull-down experiments indicated that PSMC5 interacted with ERβ in vitro. The transcriptional assay showed that PSMC5 enhanced ERβ-mediated transcriptional activities of breast cancer cell line ZR75-1, SKBR3 and human embryonic renal cell line 293T in a hormone-dependent manner. These data suggest that PSMC5 may be essential in breast cancer growth and progression through ERβ Signaling.

关 键 词：ERΒ PSMC5 相互作用 共调节因子 

分 类 号：Q753[生物学—分子生物学]