门静脉回流、肠造瘘式大鼠胰十二指肠移植模型的建立  

Establishment of pancreaticoduodenal transplantation model in rats with potal venous drainage and duodenostomy

作　　者：虞俊波[1] 徐泽宽[1] 

机构地区：[1]南京医科大学第一附属医院普外科,中国南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第6期393-395,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅135重点人才基金资助项目(苏卫科教[2003]19号)

摘　　要：目的:建立大鼠胰十二指肠移植模型,为胰腺移植的基础实验提供研究工具。方法:近交系SD大鼠胰腺移植。链尿佐菌素按50 mg/kg的剂量一次性尾静脉注射诱导糖尿病大鼠模型。供胰血液回流重建采用门静脉回流方式,即将附带腹腔干和肠系膜上动脉分支的供胰腹主动脉与受体腹主动脉行端侧吻合,供胰的门静脉与受体的肠系膜上静脉端侧吻合。十二指肠近端结扎,远端腹壁造瘘。结果:共进行38次大鼠胰十二指肠移植实验,其中33例术后血糖正常且存活超过7天,移植成功率为86.8%。术后1、3、5天血糖平均为(4.91±0.84)mmol/L、(5.19±1.06)mmol/L以及(5.67±1.47)mmol/L。结论:本胰腺移植模型操作简便、结果稳定,并能较好地模拟临床胰腺移植过程,适用于胰腺移植的基础实验研究。Objective： To establish a rat model of pancreaticoduodenal transplantation for experimental research. Methods： Diabetes was induced by single intravenous injection of streptozocin （STZ） at a dose of 50 mg/kg body weight.A total pancreas duodenal transplantation model was adopted. The aortic segment of graft ,including the celiac and superior mesenteric divergences, was end-to-side anastomosed with recipient abdominal aorta, while the donor portal was end-to-side anastomosed with recipient superior mesenteric vein.The proximal part of duodenum of the graft was ligated.A stoma was made on the distal end. Results： Thirtyeight couples of procedures were performed,out of which 33 survived more than 7d with normal blood glucose level.The successful rate of transplantation was 86.8%. The mean level of blood glucose 1,3 and 5 days after transplantation was （4.91 ± 0.84）mmol/L, （5.19 ± 1.06）mmol/L and （5.67 ± 1.47）mmol/L. Conclusion： This pancreaticoduodenal transplantation model provides a useful tool for experimental research on immunology and physiology of pancreas transplantation.

关 键 词：胰十二指肠移植 门静脉回流 造瘘 糖尿病模型 大鼠 

分 类 号：R622.9[医药卫生—整形外科]