脉心康胶囊对载脂蛋白E基因敲除小鼠肝细胞内Ca^(2+)及线粒体膜电位的影响  

Effect of Maixinkang Capsule on Ca^(2+) and Mitochondrial Membrane Potential in Liver Cells of ApoE(-/-) Mice

作　　者：郑广娟[1] 张文高[1] 朱庆均[1] 杨勇[1] 张丹[1] 王显刚[1] 季旭明[1] 

机构地区：[1]山东中医药大学基础医学院,济南250014

出　　处：《中国中西医结合杂志》2006年第5期427-430,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30472275);山东省自然科学基金资助项目(No.Y2001C14)

摘　　要：目的观察脉心康胶囊对载脂蛋白E基因敲除〔ApoE(-/-)〕小鼠肝细胞内Ca2+浓度及线粒体膜电位的影响。方法采用胶原酶消化法分离ApoE(-/-)小鼠肝细胞,体外原代培养8天后,加入10%含药大鼠血清的培养液,48h后以Flou-3和Jc-1为探针,应用激光共聚焦显微镜测量肝细胞内Ca2+浓度和线粒体膜电位。结果脉心康可明显降低ApoE(-/-)小鼠肝细胞内Ca2+浓度,与生理盐水及洛代他汀组比较差异有显著性(P<0·01);同时,脉心康可明显提高ApoE(-/-)小鼠肝细胞线粒体膜电位(P<0·01),脉心康的这两种作用无明显的剂量效应关系。结论脉心康可降低ApoE(-/-)小鼠肝细胞内Ca2+浓度,提高线粒体膜电位,从而调节脂代谢、对抗高脂血症及动脉粥样硬化的发生和发展。Objective To observe the effect of Maixinkang Capsule （MXK） on Ca^2＋ concentration and mitochondrial membrane potential in liver cells of ApoE（-/-） mice, Methods Liver cells from ApoE（-/-） mice were separated using collagenase digestive method. After the primary cells were cultured for 8 days in vitro, the concentration of 10 % MXK contained rat＇s serum was added into the culture fluid, The Ca^2 ＋ concentration and mitochondrial membrane potential in liver cells after 48-hr culture were measured by confocal laser scanning microscopy with Flou-3 and Jc-1 as probes. Results MXK could decrease Ca^2＋ concentration in liver cells, which was significantly different to that in the control group （P 〈 0.01）. Meanwhile, MXK could significantly improve mitochondrial membrane potential in liver cells （P 〈 0.01 ）, There was no obvious dose-effect relationship shown in both effects of MXK, Conclusion MXK can decrease Ca^2 ＋ concentration and improve the mitochondrial membrane potential in liver cells of ApoE（-/-） mice so as to regulate the lipids and prevent the occurrence and development of hyperlipemia and atherosclerosis.

关 键 词：脉心康胶囊 载脂蛋白E 肝细胞 线粒体膜电位 钙离子 

分 类 号：R285.5[医药卫生—中药学]