MnCl_2对树突状细胞调节T细胞抗肿瘤活性的实验研究  

Study on the antitumor activity of T cells regulated by the MnCl_2-stimulated DC

作　　者：黄少萍[1] 杨宁[1] 刘艳[1] 花菊兰[1] 

机构地区：[1]东南大学基础医学院人体解剖学与组织胚胎学系,江苏南京210009

出　　处：《东南大学学报（医学版）》2006年第3期178-181,共4页Journal of Southeast University(Medical Science Edition)

基　　金：铁道部科技基金资助项目(J99Z218)

摘　　要：目的:探讨不同浓度MnC l2对树突状细胞(DC)调节T细胞抗肿瘤活性的影响。方法:采用析因实验设计,分析MnC l2浓度、DC细胞浓度、效靶比三因素各水平对DC调节T细胞抗人肝癌细胞株(Huh7)活性的影响;流式细胞仪检测MnC l2最佳刺激组与空白组DC膜分子HLA-DR表达;体内实验分析MnC l2最佳刺激组DC在SC ID小鼠体内对T细胞抗Huh7活性的调节作用。结果:MnC l2浓度、DC细胞浓度、效靶比三因素各水平对T细胞杀伤活性皆产生显著效应(P<0.01),并且3个因素中两两有协同作用(P<0.01)。最佳MnC l2刺激浓度为300μmol.L-1,该浓度MnC l2能显著提高DC表面HLA-DR表达水平及阳性细胞率(P<0.01)。在SC ID小鼠体内,MnC l2刺激组DC成瘤率为2/5,对照组成瘤率为5/5;MnC l2刺激组的肿瘤平均体积为0.42 cm3,对照组为2.95 cm3。刺激组的成瘤率及肿瘤平均体积皆小于对照组(P<0.01)。结论:300μmol.L-1MnC l2能显著提高DC表面HLA-DR表达水平及阳性细胞率;MnC l2激活的DC在体外能显著增强T细胞杀伤活性并在SC ID小鼠体内能显著抑制肿瘤生长。Objective To investigate the effect of MnCl2 （0,150,300 μmol · L^-1）on the antitumor activity of human peripheral blood dendritic cells（DC）. Methods Factorial design and orthogonal design were adopted to analysis the effect of MnCl2 （0,150,300 μmol · L^-1 ）, DC（2 × 10^3, 1 × 10^4 ,2 × 10^4ml^ -1 ） ,E/T（5： 1,10： 1,20： 1 ） on the anfitumor activity of DC ; flow cytometry was applied to examine the expression of HLA-DR on the groups of MnCl2-stimulated DC and the non- MnCl2-stimulated DC. The effect of the best stimulated -DC by the MnCl2 （300 μmol · L^-1 ） on the cytotoxicity of T cells in SCID micet attacked by hepatic tumor cells （Huh7 ）was studied. Results These three factors all had a remarkable effect on the antitumor activity of DC. Both the expression of HLA-DR and the positive percentage of HLA- DR of MnCl2-stimulated DC were much higher than that of non-MnCl2-stimulated DC. The occurrence rate and the general volume of tumor in the SCID group injected with MnCl2-stimulated DC were remarkably less than that of the other. group injected with non- MnCl2-stimulated DC. Conclusion MnCl2 can remarkably enhance the expression and positive percentage of HLA- DR in DC in vitro. MnCl2-stimulated DC can remarkably augment the cytotoxicity of T cells and remarkably suppress the growth of tumor（ Huh7 ） in SCID.

关 键 词：人 外周血 树突状细胞 氯化锰 肿瘤 小鼠 

分 类 号：R329.23[医药卫生—人体解剖和组织胚胎学] R965[医药卫生—基础医学]