实时荧光定量PCR方法检测人、鼠正常组织及脑癌组织中CD109蛋白的表达  

Analysis of Expression of CD109 in Normal Human and Murine Tissues and Brain Cancer Tissue by Real-time Quantitative PCR

作　　者：张静敏[1] 金宁一[2] 连海[2] 李宵[2] 孙迎春[2] 安汝国[1] 高桥雅英[3] 

机构地区：[1]吉林大学药学院,长春130021 [2]军事医学科学院军事兽医研究所,长春130062 [3]名古屋大学医学部

出　　处：《中国生物制品学杂志》2006年第3期249-251,共3页Chinese Journal of Biologicals

摘　　要：目的分析CD109基因在正常组织及脑癌组织中的分布。方法采用实时荧光定量PCR方法,分析CD109在人、鼠正常组织及脑癌中的表达,以18SrRNA作为内标。结果CD109蛋白的mRNA丰度在睾丸组织中最高,在其它组织中较低,在12份脑癌标本中,检测到9份CD109表达上调。结论CD109基因在多数正常组织中表达很少,有可能是治疗恶性肿瘤的一个潜在的分子靶点。Objective To analyze the distribution of CD109 gene in normal and brain cancer tissues. Methods Analyze the expression of CD109 in normal human and marine tissues as well as brain cancer tissue by real-time quantitative PCR,using 18S rRNA as an internal standard. Results The expression level of mRNA of CD109 protein was the highest in testis tissue but was low in the other tissues. However,the expression was up-regulated in 9 of the 12 brain cancer specimens. Conehtsion CD109 gene was rarely expressed in most normal tissues. It might be a potential molecular target for the treatment of malignant tumors.

关 键 词：CD109 实时荧光定量PCR 正常组织 脑癌 

分 类 号：R512.62[医药卫生—内科学] R445.2[医药卫生—临床医学]