LHRH-PE40的免疫原性及其抗体对细胞毒作用的影响  

Immunogenicity of LHRH-PE40 and Influence of Antibody against LHRH-PE40 on Cytotoxicity

作　　者：吴广谋[1] 张国利[1] 岳玉环[1] 何畅[1] 孟锐奇[1] 李俊植[1] 朱平[1] 

机构地区：[1]军事医学科学院军事兽医研究所,长春130062

出　　处：《中国生物制品学杂志》2006年第3期252-254,共3页Chinese Journal of Biologicals

基　　金："国家863"(KF028B22W210201);"中小企业创新基金"(03C26212200869);"吉林省科技发展计划重点项目"(20020405)资助.

摘　　要：目的观察连续静脉注射重组毒素LHRH-PE40所引起的家兔抗LHRH-PE40抗体产生的规律以及该抗体对细胞毒作用的影响。方法将家兔分为3组,第1组隔日连续静脉注射LHRHPE40,第2组用弗氏佐剂乳化的LHRHPE40皮下注射为阳性对照组,第3组静脉注射人血白蛋白为阴性对照组。用ELISA检测血清中的抗体水平,XTT检测LHRH-PE40与血清中和后对Hela细胞的细胞毒作用。结果抗LHRHPE40抗体水平随静脉注射时间的延长而升高,8d可以检测到抗LHRH-PE40抗体,在24d基本达到最高水平。血清中和后LHRHPE40对Hela细胞的半数抑制量较阴性血清提高1～2倍,而阳性血清较阴性血清提高14倍。结论连续静脉注射LHRH-PE40产生较低水平的抗体,这种抗体未造成LHRHPE40对Hela细胞半数抑制量产生严重影响,这为临床连续使用LHRH-PE40提供了依据。Objective To study the LHRH-PE40 antibody production induced by continuously intravenous injection with LHRH-PE40 in rabbits and the influence of the antibody on cytotoxicity. Methods The test rabbits were divided into three groups and intravenously injected every other day with LHRH-PE40, Freund adjuvant plus LHRH-PE40 （positive control） and human serum albumin（negative control） respectively. Determine the LHRH-PE40 antibody level in sera by ELISA, and the cytotoxicity of LHRH-PE40 to Hela cells by XTr method. Results The LHRH-PE40 antibody was detected 8 d and reached a peak value 24 d after injection. After incubation with serum,the iC5o of LHRH-PE40 to Hela cells increased slightly and was 1-2 times higher than that of negative control. However,the IC50 of positive control was 14 times higher than that of negative control. Conclusion Continuously intravenous injection with LHRH-PE40 induced low antibody level which showed no significant influence on the IC50 of LHRH-PE40 to Hela cells, it provided a basis for continuous administration of LHRH-PE40 in clinic.

关 键 词：LHRH-PE40 免疫原性 抗体 细胞毒性 

分 类 号：R979.1[医药卫生—药品] R971.3[医药卫生—药学]