浙江省2005年麻疹病毒流行株基因特性分析  

Analysis on the genetic characteristics of the wide-type measles virus circulating in Zhejiang province in 2005

作　　者：冯燕[1] 严菊英[1] 卢亦愚[1] 史雯[1] 茅海燕[1] 周敏[1] 余蓓蓓[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310009

出　　处：《中华流行病学杂志》2006年第5期406-408,共3页Chinese Journal of Epidemiology

摘　　要：目的分析浙江省2005年麻疹病毒流行株的基因特性。方法对暴发疫情中分离的 4株麻疹病毒采用逆转录-聚合酶链反应(RT-PCR)扩增血凝素蛋白(H)和核蛋白(N)基因,纯化产物进行核苷酸序列测定。结果浙江省2005年分离到的4株麻疹病毒流行株均为麻疹H1基因型,属于中国麻疹病毒优势基因型。各分离株之间H基因氨基酸同源性为99．2％-99．7％,N基因氨基酸同源性为99．8％;与中国疫苗株沪191株的H基因和N基因比较,其氨基酸水平上的同源性分别为 95．2％-95．5％和95．5％。结论浙江省2005年分离到的麻疹病毒流行株属于同一性状毒株,均为 H1基因型;与中国疫苗株沪191株相比较,两者在基因特性上存在明显差异。Objective To study the genetic characteristics of measles viruses circulating in Zhejiang province in 2005. Methods 4 groups of measles viruses isolated in outbreaks and the H and N gene were amplified by reverse transcription polymerase chain reaction （RT PCR）. PCR products were purified, sequenced and data was analyzed. Results All of the 4 measles isolates belonged to genotype H1 which had been a main genotype containing all of the isolates in China. The isolates shared 99.2 % 99.7 % identity of amino acid sequence on H and 99.8 96 identity of amino acid sequence on N gene. When comparing to the China vaccine strain （Shanghai 191 ）, there were 95.2 96 -95.5 96 homogeneties and 95.5 % homogeneties on H and N gene respectively. Conclusion Data from phylogenic trees of H and N gene revealed that the wide-type measles viruses circulating in Zhejiang province in 2005 all belonged to genotype H1. There were obvious differences on genetic characteristics between the isolates and the genotype A （Shanghai 191 ）.

关 键 词：麻疹病毒 基因型别 核苷酸序列 

分 类 号：R181.8[医药卫生—流行病学]