丹参酮对爪蟾卵母细胞外源清道夫受体-AⅠ基因表达的影响  

Effect of Tanshinone on Expression of Scavenger Receptor-AⅠin Xenopus Oocytes

作　　者：高丽萍[1] 沃兴德[1] 范春雷[1] 宋家梁[1] 严瑾 李东成[2] 

机构地区：[1]浙江中医药大学生命科学系,杭州310053 [2]浙江中医药大学药学院,杭州310053

出　　处：《中国药学杂志》2006年第10期744-747,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金(30070932)

摘　　要：目的从基因调控水平探讨丹参酮对清道夫受体AⅠ(SR-AⅠ)基因表达的影响以及抗动脉粥样硬化的作用机制。方法将人SR-AⅠ基因构建在其调控元件SRad-SRap的下游,并经显微核注射导入爪蟾卵母细胞,建立SRad-SRap元件调控的SR- AⅠ表达系统；氧化低密度脂蛋白(OXLDL)刺激培养牛主动脉平滑肌细胞,制备平滑肌细胞条件培养液(SCM)；分别用SCM和含有丹参酮的SCM培养注射后的卵母细胞,用荧光标记配体法和ABC-Elisa法定性、定量检测卵母细胞膜上SR-AⅠ的表达。结果丹参酮可以抑制卵母细胞膜SR-AⅠ表达,且有量效关系。结论OXLDL可通过诱导平滑肌细胞产生相关因子,经SRad-SRap调控途径的作用上调SR-AⅠ的表达；丹参酮可以阻止SR-AⅠ的过量表达,可能是治疗As的作用机制之一。OBJECTIVE To investigate the role of tanshinone on the expression of Scavenger Receptor- A Ⅰ （ SR- A Ⅰ ） and the mechanism of anti-atherosclerosis.METHODS The target gene SR-A Ⅰ was cloned at the down stream of SRad-SRap regulatory elements,which was microinjected into the xenopus oocytes. These xenopus oocytes were treated with smooth muscle cell conditioned medium（SCM） or tanshinone SCM. After three days, the expression of the SR-AⅠ gene was detected by Di Ⅰ -OXLDL and ABC-Elisa seperately. RESULTS The expression of pSRad-SRap-SR-A Ⅰ on the membrane of xenopus oocytes was inhihited by tanshinone in a concentration-dependent manner. CONCLUSION SCM can up-regulate the expression of SR-A Ⅰ gene, which can be inhibited by tanshinone. This effect maybe relate to the mechanism of anti-atherosdevosis.

关 键 词：爪蟾卵母细胞 清道夫受体AⅠ 调控元件 氧化低密度脂蛋白 丹参酮 

分 类 号：R392[医药卫生—免疫学]