血清丙型肝炎病毒抗原的免疫酶斑点法检测  

Detection of HCV antigen in the serum by immunodotting

作　　者：时红波[1] 谢立[1] 黄德庄[1] 贺立香[1] 

机构地区：[1]首都医科大学附属北京佑安医院肝炎研究所,北京100054

出　　处：《细胞与分子免疫学杂志》2006年第3期336-338,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：首都医学发展科研基金课题(No.2002-3068);首都医科大学基础临床合作基金课题(No.02JL20)

摘　　要：目的建立一种可筛查早期HCV感染者的简单快捷方法。方法制备特异性的抗HCV核心蛋白和抗HCVNS3的单克隆抗体(mAb)酶标记物,应用免疫酶斑点法检测各种类型肝炎患者和正常人血清中的HCV-Ag,并与双抗体夹心ELISA检测HCV-Ag及RT-PCR检测HCVRNA的结果进行比较。结果免疫酶斑点法检测表明,HCV-Ag的检出率在抗HCV抗体阳性、乙肝和非甲非戊肝炎患者中,分别为15.4%(8/52)、1.73%(19/1099)和1.03%(1/97);在81例甲肝和67例戊肝患者及50例正常人中均未检出。免疫酶斑点法与双抗体夹心ELISA及RT-PCR的检测的结果没有显著性差异,但其与RT-PCR检测结果有一定的相关性。结论免疫酶斑点法特异性强,操作简便快捷,不需要特殊仪器设备,为HCV的早期诊断和常规筛查提供了有效的方法。AIM： To set up a simple and rapid screening method of early dection for HCV infection. METHODS： The HRP-anti-HCV monoclonal antibodies （mAbs） conjugate was prepared by the mAbs of anti-HCV-core and anti-HCV-NS3 with high specificity. HCV antigens in the sera from hepatitis patients and normal persons were detected by immunodotting and compared by double antibody ELISA and RT-PCR. RESULTS： The positive ratio for HCV antigen was 15.4%（8/52）, 1.73%（19/1099） and 1.03%（1/97） in hepatitis C patients and hepatitis B patients and hepatitis NA-NE patients, respectively. The results for HCV antigen were negative in hepatitis A patients （0/81）, hepatitis E patients （ 0/67 ） and normal persons （0/50）. There was no significant difference among the immunodotting and double antibody ELISA and RT-PCR. There was definite correlation between the immunodotting and RT-PCR. CONCLUSION： This method, simple and rapid with high specificity, provides an effective way for the early diagnosis and routine detection of HCV.

关 键 词：丙型肝炎病毒 免疫酶斑点法 抗原 单克隆抗体 

分 类 号：R373.21[医药卫生—病原生物学]