雌激素对急性心肌梗死前后去卵巢大鼠干细胞及心功能的影响  

Effects of estradiol intervention on peripheral blood CD90^+ cells and cardiac function in de-ovary rats before and after myocardial infarction

作　　者：郑小璞[1] 马爱群[1] 韩克[1] 刘宇[1] 张炜[1] 王锐[1] 

机构地区：[1]西安交通大学医学院第一附属医院心血管内科,环境与疾病相关基因教育部重点实验室心血管离子通道病研究室,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2006年第3期350-352,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：陕西省科技攻关项目(No.2002D32)

摘　　要：目的去卵巢大鼠发生急性心肌梗死前后,应用雌激素干预,观察其外周血干细胞的变化及其对心功能的影响。方法将30只SD雌性大鼠分为正常对照组、急性心肌梗死组、去卵巢心肌梗死组、去卵巢雌激素替代组及去卵巢雌激素治疗组。用流式细胞术检测各组CD90+细胞的百分率;用POWERLAB4.12系统测量左室收缩末压(LVESP)、左室舒张末压(LVEDP)、+dp/dtmax及-dp/dtmax。结果去卵巢心肌梗死组外周血CD90+细胞的百分率显著低于急性心肌塞组及去卵巢雌激素替代组(P<0.01);而去卵巢雌激素替代组心梗后第1天外周血CD90+细胞的百分率即显著升高(P<0.05),第3天达峰值,且数值较高。去卵巢雌激素治疗组与去卵巢心肌梗死组相比较,仅在心梗后7d外周血CD90+细胞的百分率才开始升高;但与去卵巢雌激素替代组相比,心梗术后7d内各时点均较低。各组LVESP均有所下降,以急性心肌梗死组为著;而LVEDP均有升高,以去卵巢心肌梗死组及去卵巢雌激素治疗组最明显(P<0.01)。结论去卵巢大鼠在心肌梗死之前应用雌激素替代治疗,外周血CD90+细胞的百分率较高,且心功能明显优于去卵巢大鼠在心肌梗死后应用雌激素替代治疗。AIM： To investigate the effects of estradiol intervention on peripheral blood CD90^＋ cells and cardiac function in de-ovary rats before and after myocardial infarction. METHODS： Thirty SD female rats were divided into the control group, acute myocardial infarction group, de-ovary myocardial infarction group, de-ovary replacement group with estradiol, and de-ovary therapy group with estradiol. CD90^＋ cell percentage was determined with flow cytometry. The left ventricle end systolic pressure （ LVESP）, left ventricle end diastolic pressure （LVEDP）, ＋ dp/dtmax and -dp/dtmax were also measured with the POWERLAB4.12 system. RESULTS： The percentage of peripheral blood CD90^＋ cells in the de-ovary myocardial infarction group was remarkably lower than that in the acute myocardial infarction group and the de-ovary replacement group with estradiol （ P〈0.01 ）. In the de-ovary replacement group with estradiol, the percentage of peripheral blood CD90^＋ cells remarkably elevated following the first day of operation （ P〈0.05 ）, reaching the peak value at clay 3. In the de-ovary therapy group with estradiol, it started to elevate at day 7 after the operation, much lower than that in the de-ovary replacement group with estradiol. LVESP in each operation group decreased, especially in the acute myocardial infarction group, whereas LVEDP increased in the de-ovary group and de-ovary therapy group with estradiol （ P 〈 0. 01 ）. CONCLUSION： Replacement with estradiol before myocardial infarction exerts more beneficial effects than estradiol replacement after myocardial infarction in de-ovary rats.

关 键 词：急性心肌梗死 干细胞 心功能 雌激素 去卵巢大鼠 

分 类 号：R542.2[医药卫生—心血管疾病]