基于溶胶-凝胶技术结合多壁纳米碳管化学修饰电极的方法制备高灵敏葡萄糖生物传感器的研究  

Study on preparation of highly-sensitive glucose biosensor based on solgel combined with multi-walled carbon nanotubes modified electrode

作　　者：黄加栋[1] 宋昭[1] 杨钰[1] 吴宝艳[1] 史海滨[1] 长哲郎[2] 陈强[1] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]日本东北大学药学部,仙台980-8578

出　　处：《高技术通讯》2006年第5期492-496,共5页Chinese High Technology Letters

基　　金：国家自然科学基金（90209054）资助项目.

摘　　要：基于多壁纳米碳管修饰铂电极与二氧化硅溶胶-凝胶（sol-gel）固定化酶相结合的技术制备了葡萄糖氧化酶传感器,充分利用了溶胶-凝胶固定化酶稳定的优点和纳米碳管的高灵敏电催化作用,优化了该酶传感器的制备过程,提高了传感器的电流响应和反应线性.结果表明,sol-gel构建的优化条件是：H2O：TEOS为2.5～3.5,TritonX-100浓度为5%,pH值为5.5.在本实验条件下,多壁纳米碳管的最适固定量为5μl（0.25g/L）,溶胶-凝胶与酶的优化体积比为3：2.工作电位＋0.55V、pH 6.5、25℃为制备传感器的最适工作条件.该传感器对葡萄糖在0.5～6 mmol/L呈线性响应,响应时间为20 s,检出限为0.05mmol/L,45天时的响应值仍保持90%.A highly-sensitive glucose biosensor was prepared based on multi-walled carbon nanotubes modified electrode combined with SiO2 sol-gel immobilization method. Its fabrication procedure, related experiment conditions and electrochemical characterization were studied in details. H2O：TEOS = 2.5 - 3.5,5% TritonX-100,pH5.5 were the optimum fabrication procedure parameters of sol-gel. 5μl MWCNTs solution（0.25g/L）, sol-gel/GOD = 3：2（v/v） were the optimum fabrication parameters of enzyme-electrode. The optimum detection potential was ＋ 0.55 V, pH 6.5 at 25 ℃. Under selected conditions the sensor offered a linear range of 0.5 - 6 mmol/L and a detection limit of 0.05 mmol/L. The response time was 20s. The sensor remained 90% of its response after using 45 days.

关 键 词：溶胶-凝胶 多壁纳米碳管 化学修饰电极 葡萄糖氧化酶 电流型生物传感器 

分 类 号：TQ127.2[化学工程—无机化工] TP212.3[自动化与计算机技术—检测技术与自动化装置]