金钱树的组织培养  

Tissue Culture of Zamioculcas zamiifolia (Loddiges) Engler

作　　者：顾丽红[1] 杨本鹏[1] 张树珍[1] 杨学[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室,海口市571101

出　　处：《热带农业科学》2006年第2期19-21,32,共4页Chinese Journal of Tropical Agriculture

摘　　要：以金钱树[Zamioculcaszamiifolia(Loddiges)Engler]的幼嫩小叶为外植体,采用幼叶→愈伤组织→丛生芽→完整植株的途径进行繁殖。结果表明:叶片在MS+BA2mg/L+2,4-D1.0mg/L+PVP0.5mg/L+活性炭0.5mg/L+蔗糖30g/L培养基上暗培养30d,可诱导形成愈伤组织;愈伤组织在MS+BA4mg/L+KT0.5mg/L+蔗糖30g/L培养基上光照培养25d,可诱导形成丛生芽(丛生芽在继代培养过程中每20～25d可增殖3～5倍);丛生芽接种于MS+BA3mg/L+NAA0.2mg/L+蔗糖30g/L培养基上壮苗培养4周后,再转入1/2MS+BA2mg/L+NAA0.5mg/L+蔗糖30g/L培养基上培养30d,可诱导形成完整的根系。Leaf explants of Zamioculcas zamiifolia （Loddiges） Engler were inoculated on MS medium containing 2 mg/L BA （6-benzylaminopurine）, 2,4-D （2,4-dichlorophenoxya-cetic acid） 1.0 mg/L, PVP （Polyvinylpyrolidone） 0.5 mg/L, AC （Active carbon） 0.5 mg/L, 30 g/L Suc for inducing callus. After being cultured in darkness for 25 days, calli were formed. They were then transferred onto the MS medium supplemented with 4 mg/L BA, 0.5 mg/L KT and 30 g/L Suc for inducing clustered buds. The clustered buds were formed after being cultured for 25 days （they were multiplied 3-5 times every 20-25 days of subculture）. After cultured on the MS medium containing 0.2 mg/L NAA, 3 mg/L BA and 20 g/L Suc for about 4 weeks, the clustered buds were transferred onto the 1/2 MS medium containing 0.5 mg/L NAA, 2 mg/L BA and 30 g/L Suc for roofing. This cultural system is very useful for commercial proliferation of Z. zamiifolia.

关 键 词：金钱树 观赏植物 组织培养 愈伤组织 丛生芽 

分 类 号：S813.1[农业科学—畜牧学]