离体培养细胞表面的扫描电镜观察  

Observations on Surface of Plant Cell Cultured in vitro by Scanning Electron Microscopy

作　　者：郭骁才[1] 蒋辉[1] 吴伯骥[1] 郑国 

机构地区：[1]中国科学院成都生物研究所,兰州大学细胞生物学研究室

出　　处：《华北农学报》1996年第1期36-43,共8页Acta Agriculturae Boreali-Sinica

摘　　要：对离体培养植物细胞（胡萝卜细胞和烟草细胞）的表面进行了扫描电镜观察。结果表明，愈伤组织表面细胞的表面上分布有颗粒和纤丝。颗粒的形状、大小及分布有各种各样，有些颗粒顶部凹陷成小坑。纤丝大多与颗粒相连，在有些细胞表面上纤丝交织成网络。对这些颗粒和纤丝的分泌活动及调节，以及颗粒和纤丝在细胞之间粘连中的作用，进行了讨论。The surfaces of plant callus cells cultured in vitro from two species, Daucus carota and Nicotiana tabacum,were studied using scanning electron micrcoscopy. A number of sphericalhemispherical protuberances or beads (papillary projections) in various sizes shapes occured on the outer surfaces of the callus cell walls. Most of the protuberances appeared with a sphereal bead on an irregular base projected on the cell wall surfaces and some of the larger bases were fused from two or more smaller ones. And adjacent projections could fuse also. Some of the spherical beads exhibited two clearly distinct regions, an upper region was distinct from the basal region by a granular appearence of margin,and a lot of the spherical beads or more flattened projections collapsed as a hollow at its top part might developed from these two part projections.A number of fibrils were seen to be associated with one or more of the projections on the surfaces of the carrot callus cell walls,and some of the cells coated with a fibrillar mat,but not all of the projections were associated with fibrils. And the fibrils or fibrillar mats were not seen on the surfaces of the more loosely packed tobacco callus cells.The projections could fuse to form bridges between adjacent cells,and the fibrils could interlace intercellularly. Those observations might be taken as some evidence of initial adhesion between opposing cells from two unrelated calli after contact. The process of wall material secretion and its control were discussed.

关 键 词：离体培养 细胞表面 壁物质分泌 细胞间粘连 

分 类 号：S336[农业科学—作物遗传育种]