高沸醇木质素及其衍生物对菠萝蛋白酶的吸附  

Adsorption of Bromelain with HBS Lignin and Its Derivatives

作　　者：张夏红[1,2] 程贤甦[1,3] 汤吉[1] 

机构地区：[1]福州大学材料科学与工程学院 [2]龙岩学院化学与材料工程系,福建龙岩364000 [3]闽江学院化学与化学工程系,福建福州350011

出　　处：《过程工程学报》2006年第1期87-90,共4页The Chinese Journal of Process Engineering

基　　金：福建省教育厅科研基金B类资助项目(编号:JB02058);福州市引进人才特别基金资助项目(编号:200405);福州大学科技发展基金资助项目

摘　　要：通过曼尼希反应和室温相分离方法合成了高沸醇木质素胺和高沸醇木质素酚衍生物,并探讨了它们对菠萝蛋白酶的吸附特性.结果表明,引入了胺基和酚羟基的高沸醇木质素衍生物对菠萝蛋白酶的吸附性能有很大的提高,吸附能力为HBS木质素胺>HBS木质素酚>HBS木质素,而且吸附后的菠萝蛋白酶活性仍保持在较高的水平,高沸醇木质素衍生物有望成为菠萝蛋白酶的浓缩吸附剂或固定化的载体.Amine and phenol derivatives of high boiling point solvent （HBS） lignin were prepared by Mannich reaction and phenolic reaction, respectively. The bromelain adsorption with HBS lignin and its derivatives was investigated. The results showed that the adsorption abilities of amine and phenol derivatives of HBS lignin were improved greatly. The order of their adsorption capacities to bromelain was as follows： HBS lignin amine〉HBS lignin phenol〉HBS lignin. The activity of the bromelain was kept at a high level after the adsorption. The HBS lignin and its derivatives could be used as new concentrated adsorbents of bromelain or new carriers in bromelain immobilization.

关 键 词：高沸醇木质素 高沸醇木质素胺 高沸醇木质素酚 菠萝蛋白酶 吸附 

分 类 号：Q814.1[生物学—生物工程]