熟地和制首乌多糖对贫血小鼠骨髓有核细胞数和细胞周期的影响  

Effct of Rehmannia glutinosa and Polignum.multiflorum Thunb Polysaccharide on number and cell cycle of bone marrow cells in anemic mice

作　　者：冯雪梅[1] 吕艳[2] 祝彼得[1] 刘啸[1] 

机构地区：[1]成都中医药大学基础医学院,四川成都610075 [2]四川大学华西基础与法医学院,四川成都610041

出　　处：《四川中医》2006年第6期17-19,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金(项目批准号:30271661)

摘　　要：目的:观察熟地多糖(RPS)和制首乌多糖(PPS)对骨髓抑制贫血小鼠外周血象、骨髓有核细胞(BMC)数和细胞周期的影响。方法:用60Co辐射和腹腔注射环磷酰胺(Cy)及氯霉素(Ch),建立骨髓抑制贫血小鼠模型,分别提取熟地和制首乌多糖,腹腔注射RPS(20mg/kg)和PPS(25mg/kg),观察对外周血象、BMC数的影响,并用流式细胞仪分析细胞周期。结果:RPS和PPS均能一定程度促进外周血象的恢复,明显提高骨髓抑制贫血小鼠BMC数量(P<0.01);流式细胞术分析表明,G0/G1期细胞比例明显减少(RPS:P<0.01,PPS:P<0.05),S期细胞比例明显增加(RPS:P<0.01,PPS:P<0.01)。结论:RPS、PPS能促进骨髓抑制贫血小鼠骨髓抑制的恢复,促进骨髓有核细胞进入增值周期。Objective： To research the effect of Rehmannia glutinosa and Polignum. multiflorum Thunb polysaccharide on hemogram, the number and cell cycle of bone marrow nucleated cells （BMC） in anemic mice. Methods ： Anemic mice model resulted from myelosuppression by irradiation and cytotoxic chemotherapeutic compounds. Extract the polysaceharide from Rehmarmia glutinosa and Polignum. multiflorum Thunb. Anemic mice were treated with RPS （20mg/kg） and PPS 125mg/kg） by celiac injection. Count the numbers of peripheral blood cells and bone marrow nucleated cells; analyze the cell cycle by Flow Cyto Merry （FCM） of bone marrow nucleated cells in anemic and treated mice. Results ： RPS and PPS can increase the numbers of peripheral blood cells to some degree and the number of BMC markedly （ P 〈 0.01 ） in anemic mice; RPS and PPS also can decrease the number of BMC in G0/G1 phase （RPS： P 〈 0.01, PPS： P 〈 0.05） and increase the number in S phase （RPS： P 〈 0.01, PPS： P 〈 0.05） evidently. Conclusions ： RPS and PPS can promote the recovery of myelosuppression and promote the bone marrow nucleated cells to enter the cell cycle.

关 键 词：贫血小鼠 熟地总多糖 制首乌总多糖 骨髓有核细胞 细胞周期 

分 类 号：R285.5[医药卫生—中药学] R259.546[医药卫生—中医学]