鼻息肉组织中IL-4 IL-5 IL-6和IL-8的含量及其表达  

The concentration and expression of IL-4,IL-5,IL-6,IL-8 in human nasal polyps tissues

作　　者：乐建新[1] 陈建军[1] 孔维佳[1] 檀惠芳[1] 舒宏[1] 施秋梅[1] 

机构地区：[1]华中科技大学协和医院耳鼻咽喉科,武汉430022

出　　处：《临床耳鼻咽喉科杂志》2006年第11期484-486,共3页Journal of Clinical Otorhinolaryngology

基　　金：湖北省自然科学基金资助项目(No:2000J067)

摘　　要：目的:通过检测鼻息肉组织中IL4、IL5、IL6、IL8的水平及表达方式,探讨细胞因子在鼻息肉形成中的作用。方法:应用放射免疫法检测54例鼻息肉组织(鼻息肉组)中IL4、IL5、IL6及IL8的浓度,同时用免疫组织化学法观察其表达,以22例行鼻中隔手术患者中鼻甲黏膜作为对照(对照组)。结果:鼻息肉组IL5及IL8水平均明显高于对照组(均P<0.01);而IL6在两组间差异无统计学意义(P>0.05);IL4在变应原皮试阳性组明显增加,与对照组比较P<0.05。IL4主要表达于息肉组织内炎性细胞,多为淋巴细胞或浆细胞;IL5主要表达于鼻息肉组织中嗜酸粒细胞和淋巴细胞;IL6、IL8主要表达于息肉上皮层及息肉组织中的炎性细胞皮试阳性组和阴性组间无明显区别。结论:IL5及IL8在所有鼻息肉组织中具有一定作用,而IL4仅在变应原皮试阳性息肉中具有一定意义,IL6在鼻息肉组织中无明显作用。Objective ： To study the concentration and expression of IL-4, IL-5, IL-6, IL-8 in human nasal polyps tissues, and to explore the relationship between cytokines and the formative mechanism of nasal polyps. Method：The concentration and expression of IL-4,IL-5,IL-6,IL-8 in 54 cases with nasal polyps were determined by enzyme linked immunosorbent assay（ELISA） and immunohistochemistry, and the middle turbinate mucosa from 22 patients with the deviation of nasal septum were used as the control. Result： The concentration of IL-5, IL-8 in nasal polyps tissues were significantly higher than that in normal middle turbinate mucosa（ P 〈0.01）. There was no significant difference between the concentration of IL-6 in nasal polyps and that in normal control group（ P 〉0.05）. No significant difference was found between the concentration of IL-4 in nasal polyps tissues with negative allergic skin test and that in control group, but the concentration of IL-4 was significantly higher in nasal polyps tissues with positive allergic skin test than in control group（ P 〈0.05）. IL-4 was mostly found in lymphocytes and plasma cells in nasal polyps and IL-5 in eosinophils and lymphocytes. IL-6 and IL-8 were mostly found in nasal polyps epithelium and inflammatory cells, as was no difference between positive allergic skin test and negative one. Conclusion：IL-5 and IL-8 are the key cytokines in the formation of nasal polyps, and IL-4 is a key cytokine in nasal polyps with positive allergic skin test. IL-6 isnt an important cytokine in the formative mechanism of nasal polyps.

关 键 词：鼻息肉 白细胞介素 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]