载脂蛋白AⅠ基因多态性与老年人群血脂水平关系的研究  

Study on relationship between apolipoprotein AⅠ gene polymorphisms and plasma lipids in the aged

作　　者：王延坤[1] 赵斐[2] 张勇[2] 刘艳环[2] 姜宁[2] 张薇[2] 

机构地区：[1]武警医学院军事预防医学研究所 [2]天津体育学院运动医学研究所,天津300381

出　　处：《中国老年学杂志》2006年第5期577-580,共4页Chinese Journal of Gerontology

基　　金：国家体育总局应用基础项目(21021);天津市教委科技发展基金(01-21705)

摘　　要：目的探讨载脂蛋白AⅠ(ApoAⅠ)基因多态性与老年人血脂水平的关系。方法应用多聚酶链反应和限制性片段长度多态性技术(PCR-RFLP)确定ApoAⅠ基因启动区-75 bp位点和第一内含子区+83 bp位点基因多态性,并测定血脂各项指标。结果血浆ApoAⅠ、HDL-C水平在-75 bp位点不同基因型组间变化趋势为M1--组>M1+-组>M1++组,其中M1++组、M1+-组ApoAⅠ、HDL-C显著低于M1--组,且此种组间差异仅在女性中存在。+83 bp位点不同基因型组间血脂指标未见显著差异。结论ApoAⅠ基因启动区-75 bp位点多态性与血浆ApoAⅠ、HDL-C水平存在一定关联,该位点G/A转换与高水平ApoAⅠ、HDL-C有关。Objective To investigate the relationship between apolipoprotein A Ⅰ （ApoA Ⅰ ） gene polymorphism and serum lipids in Chinese aged people. Methods PCR-RFLP methods were used to detect the ApeA Ⅰ gene -75 bp and ＋83 bp site genotype,and serum lipids and apelipeproteins were measured. Results The levels of serum ApeA Ⅰ and HDL-C in -75 bp site genotype were M1 - - group 〉 M1 ＋ -group 〉M1 ＋ ＋group,and people with M1 - - genotype had significantly higher ApeA Ⅰ ,HDL-C levels than those with M1 ＋ ＋ and M1 ＋ -genotype, but these differences were detected only in women. Serum lipids and apelipeproteins were similar in different genotype groups of ＋ 83 bp site. Conclusions ApeA Ⅰ -75 bp site pelymorphism is associated with serum ApeA Ⅰ and HDL-C levels,and at this site G/A transition is related to the increased ApoA Ⅰ and HDL-C levels.

关 键 词：载脂蛋白AⅠ 基因多态性 血脂 

分 类 号：R589.2[医药卫生—内分泌] Q754[医药卫生—内科学]