高效液相色谱法测定人血浆奥卡西平代谢产物浓度  

Determination of the Concentration of the Metabolite of Oxcarbazepine in Human Blood Plasma with HPLC

作　　者：刘伟忠[1] 黄伟侨[2] 王广发[1] 付燕[1] 温预关[1] 

机构地区：[1]广州市精神病医院国家临床药理研究基地,510370 [2]中山大学附属第一医院药学部,广州510080

出　　处：《医药导报》2006年第6期504-506,共3页Herald of Medicine

基　　金：广东省社会发展领域科技计划项目基金(编号:53060)

摘　　要：目的 建立测定人血浆奥卡西平代谢产物浓度的高效液相色谱法.方法 以C18反相柱（150 mm×4.6 mm,5 μm）为色谱柱,流动相为甲醇-水（55：45）,流速为1 mL·min^-1,检测波长254 nm,以乙酸乙酯为提取剂.结果奥卡西平代谢物的高、中、低（120.0,60.0,12.0 mg·L^-1）3种浓度平均回收率分别为100.43%,99.33%,99.17%,日内、日间差RSD均＜6%（n＝5）; 分析方法的最低检测浓度为6.0 mg·L^-1;线性范围为12.0～120.0 mg·L^-1.回归方程为：C＝77.06F＋1.22,r＝0.9996（n＝10）.结论 该方法灵敏、准确、简单、快速,可用于血浆奥卡西平代谢物浓度监测和药动学研究.Objective To set up an HPLC method for the determination of the concentration of the metabolite of oxcarbazepine in human blood plasma. Methods Blood plasma samples containing the drug were extracted with hexane for the removal of lipids and other interfering substances and thereafter extracted with ethyl acetate. The extract was then analyzed in a reverse phase HPLC system（ C18 column, 150 mm× 4.6 mm, 5μm） ; mobile phase, methanol-water（55 ： 45） ; UV detection wavelength,254 nm）. Results The average rates of,recovery of the metabolite of oxcarbazepine in high , medium and low concentrations （ 120.0,60.0,12.0mg·L^-1 ） were 100.43%, 99.33% and 99.17%, respectively. The inter-day and intra-day relative standard deviations were less than 6% （n = 5 ）. The calibration curve had a good linearity r = 0.999 6 （n = 10 ） when the metabolite was within a concentration range of 12.0 - 120.0 mg·L^-1. The regression equation was C = 77.06F ＋ 1.22, r = 0. 999 6 （ n = 10 ）. The minumum detectable concentration of the metabolite of oxearbazepine was 6.0 mg mg·L^-1. Conclusion The method was shown to be sensitive,accurate,handy and speedy and may be used in the monitoring of the blood concentration of oxcarbazepine and phamacokinetic studies of the drug.

关 键 词：奥卡西平 代谢产物 高效液相色谱法 

分 类 号：R971.6[医药卫生—药品] R969.1[医药卫生—药学]