白藜芦醇苷对急性心肌梗死模型犬的影响  

Effect of Piceid on the Dog with an Acute Myocardial Infarction Model

作　　者：张冬齐[1] 孙莉莎[1] 徐江平[1] 

机构地区：[1]南方医科大学药学院药理学教研室,广州510515

出　　处：《医药导报》2006年第6期510-513,共4页Herald of Medicine

摘　　要：目的观察白藜芦醇苷注射液对急性心肌梗死模型犬的影响。方法将犬麻醉后,采用冠状动脉左前降支结扎造成心肌梗死模型,分别给予白藜芦醇苷注射液2.5,5.0,10.0 mg.kg-1,测定犬心肌梗死范围并检测犬血清乳酸脱氢酶(LDH)、肌酸激酶(CK)的活性。结果白藜芦醇苷静脉注射可显著降低心肌梗死模型犬心肌缺血的程度,缩小心肌缺血的范围;显著减小梗死范围,降低血清LDH、CK升高的程度;减轻心肌缺血时的细胞损害。结论白藜芦醇苷注射液对急性心肌梗死模型犬心肌细胞有明显的保护作用。Objective To survey the effect of piceid on dogs with a model of acute myocardial infarction. Methods 30 healthy mongrel dogs weighing 10 - 15 kg served as the subjects of the study. The model of acute myocardial infarction was established by ligation of the left anterior descending branch of the coronary artery in the dogs under anaesthesia and mechanical ventilation. 10 minutes after the ligation the dogs were given each piceid in low （2.5 mg·kg^-1 ,n =6） , medium （5.0 mg·kg^-1, n =6） and high（ 10. 0 mg·kg^-1, n = 6） doses, respectively, administered by Ⅳ instillation lasting 30 rain. Animals of the solvent control group （ n = 6） were given each an equivalent volume of the solvent in the same manner while those of the blank control group （n = 6 ） were given no treatment, The severity of myocardial ischemia was reflected by the epicardial electrocardiogram. Blood samples from the right ventricle were taken before and 2 h after the medication for the determination of the activities of the serum lactate dehydrogenase（LDH） and creatine kinase （CK）. 2 h after the ligation of the artery, the dogs were sacrificed and the hearts taken for the determination of the extent of infarction with the NBT staining method. Results Intravenous instillation of piceid was shown to significantly decrease the severity of myocardial ischemia and reduce the extent of infarction in a dose-dependent manner. The drug was also found to mitigate the increase in the activities of serum LDH and CK. Conclusion Piceid injection was shown to have striking protective effects on dogs with acute experimental myocardial infarction.

关 键 词：白藜芦醇苷 心肌梗死 乳酸脱氢酶 肌酸激酶 

分 类 号：R285.5[医药卫生—中药学] R972[医药卫生—中医学]