反相高效液相色谱法测定甘露消毒丸中马兜铃酸Ⅰ、Ⅱ的含量  

Determination of the Content of Aristolochic Acid Ⅰand Ⅱ in Ganluxiaodu Pills with RP-HPLC

作　　者：朱光辉[1] 王奇志[2] 王增寿[1] 苏孝共[1] 

机构地区：[1]温州医学院附属第二医院药剂科,325027 [2]温州医学院药学院药理教研组,325003

出　　处：《医药导报》2006年第6期568-570,共3页Herald of Medicine

基　　金：温州市科技局科研基金项目(基金编号:Y2004A074)

摘　　要：目的建立反相高效液相色谱法测定甘露消毒丸中马兜铃酸Ⅰ、Ⅱ的含量。方法采用C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水-冰醋酸(68∶32∶1)为流动相,检测波长为250 nm,流速为1.0 mL.m in-1。用超声提取法提取甘露消毒丸中的马兜铃酸Ⅰ、Ⅱ。结果马兜铃酸Ⅰ在0.135~27.000μg.mL-1(r=0.999 8,n=5),马兜铃酸Ⅱ在0.325~65.000μg.mL-1(r=0.999 9,n=5)线性关系良好。马兜铃酸Ⅰ、Ⅱ的最低检测浓度分别为0.081,0.065μg.mL-1,平均回收率分别为98.50%,99.12%,RSD分别为2.30%,3.50%。结论该方法简便、快速、灵敏。Objective To set up a reversed-phase HPLC method for the determination of aristolochic acid Ⅰand Ⅱ in ganluxiaodu pills. Methods A C_(18) chromatographic column(4.6 mm×250 mm,5 μm) served as the solid phase;methanol-water-acetic(68∶32∶1) was the mobile phase;the flow rate,1.0 mL·min^(-1);UV detection wavelength,250 nm.The aristolochic acids Ⅰand Ⅱ in the ganluxiaodu pills were extractad with the ultrasonic extraction method. Results There was a good linear relationship over a range of 0.135-27.000 μg ·mL^(-1) for aristolochic acid Ⅰ(r=0.999 8,n=5) and(0.325-65.000) μg·mL^(-1) for aristolochic acid Ⅱ(r=0.999 9,n=5).The lowest detection concentrations of aristolochic acid Ⅰ and Ⅱ were 0.081 μg·mL^(-1) and 0.065 μg·mL^(-1),respectively.The average rates of recovery of aristolochic acid Ⅰ and Ⅱ were 98.50% and 99.12%,respectively,the RSD were 2.30% and 3.50%,respectively. Conclusion The method was shown to be handy,rapid and sensitive.

关 键 词：甘露消毒丸 高效液相色谱法 反相 马兜铃酸Ⅰ 马兜铃酸Ⅱ 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]