骨组织工程支架材料与新骨生长研究  

Tissue Engineering Stent Material and New Bone Growth

作　　者：田杰谟[1] 董利民[1] 王晨[1] 郭志平[1] 张朝宗[1] 王贤刚[1] 卢世璧[2] 孙明学[2] 彭江[2] 

机构地区：[1]清华大学新型陶瓷与精细工艺国家重点实验室,北京100084 [2]中国人民解放军总医院,北京100084

出　　处：《中国医疗器械信息》2006年第5期5-8,共4页China Medical Device Information

基　　金：国家自然科学重点基金资助(项目号:30330570)

摘　　要：本文对β-TCP、β-TCP/DCHA和HA材料的结构、表面生物活性及细胞在材料表面的粘附情况进行了检测与分析,用Micro-CT对制备的骨支架材料植入前后的结构进行了评价。将骨髓基质细胞进行培养、分化,扩增得到足够浓度的成骨细胞,并种植于骨支架材料复合培养后,再植入动物体,观察成骨状况, 研究成骨机制。结果表明:骨支架的孔隙中诱导生成新骨,随着新骨生长,支架材料在逐步降解,从而达到骨重建。研制出了具有三维多孔结构、适于新骨生长的孔径、可降解、表面生物活性好以及植入骨细胞后可以诱导新骨生成骨组织工程支架材料。This paper deseribes the structures of b-TCP,b-TCP/DCHA and HA,tests and analysis for their surface bioactivity and osteoblasts attachment and the evaluation structure before or after implantaion used by Micro-CT test. The bone marrows stromal cells were cultured,or separation and proliferation were made to reach up to osteoblasts concentration of 10^5/cm^3,and then the osteoblasts were immersed in the biomaterials and implated in the rabbits.The bone formation was observed and the machinism of new bone formation was investigated.The experimental results after implantation show that introducing new bone grows into the scaffold holes,and scaffolds were degraded with new bone formation and growth.And the end,the bone were reconstructed.The results indicate these tissue engineering scaffolds possesses 3-D porous structure,pore size appropriate new bone growth,degradable,high bioactive and inducing new bone formation.

关 键 词：骨组织工程 支架材料 三维结构 生物活性 Β-磷酸三钙 类骨磷灰石 

分 类 号：R318.08[医药卫生—生物医学工程]