一起食物中毒金黄色葡萄球菌肠毒素基因的检测  

Detection of staphylococcal enterotoxin genes in Staphylococcus aureus isolates from food poisoning

作　　者：王赞信[1] 张俊彦[1] 朱敏[1] 张严峻[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310009

出　　处：《中国卫生检验杂志》2006年第6期665-666,688,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:检测从食物中毒中分离的金黄色葡萄球菌的肠毒素基因,明确肠毒素的基因型。对所分离的金黄色葡萄球菌进行药敏试验,检测其耐药性。方法:应用PCR扩增金黄色葡萄球菌SEA-SEJ基因,电泳检测扩增结果;用VITEK32检测药敏结果。结果:检测到金黄色葡萄球菌的肠毒素基因SEG和SEI,菌株的耐药性不强。结论:金黄色葡萄球菌的肠毒素SEG和SEI可能是这起食物中毒的因子。Objective： To detect and type staphylococcal enterotoxin in Staphylococcus aureus isolates from food poisoning. Methods：Toxin genes were amplified by PCR, and the different amplicons in size detected by eleetrophoresis. The antibiosis were determined by VITEK32. Results： Entevo genes SEG and SEI were detected in S. aureus isolates. Conclusion： The SEG and SEI are possible causes for this food poisoning.

关 键 词：金黄色葡萄球菌 肠毒素 基因 PCR 

分 类 号：R378.11[医药卫生—病原生物学]