复方毛冬青注射液中甲基正壬酮的含量测定  

Determination of 2-undecanone in compound Maodongqing injection by GC

作　　者：邓六勤[1] 钟鸣 陈怡禄[1] 何艳玲[1] 黄跃[1] 卓仪[1] 

机构地区：[1]广东省广州市儿童医院,广东广州510120 [2]广州汉方现代中药研究开发有限公司,广东广州510240

出　　处：《儿科药学杂志》2006年第3期42-44,共3页Journal of Pediatric Pharmacy

基　　金：广州市卫生局资助项目(2005A027;30307-3200516)

摘　　要：目的：为复方毛冬青注射液建立专属性含量测定方法。方法：采用气相色谱法对甲基正壬酮进行含量测定。色谱柱：HP-1（Aglient 19091z-413，30m×320μm×0.25μm）；氮气为9.23psi，流量1.8mL/min；空气400mL／min；氢气40mL/min；柱温80℃（2min）至220℃，升温速率为15℃/min；再升至270℃（13.75min），升温速率为40℃／min；汽化室240℃；检测室280℃；进样量1μL；检测器为FID。结果：线性范围为1.0—20.0μg／mL，平均回收率为99.05％，RSD=1.29％（n=6）。结论：本法操作简单、可靠、具有实用性。Objective：To establish a quality control method for compound Maodongqing injection. Methods：The 2-undecanone was determined by GC. Chromatographic conditions were Air： 400 mL/min; Hydrogen： 40 mL/min; HP-1 （Agilent, USA, 30 minx0.25 mm I.D, 0.25 μm film thickness） ; Column temperature： 80 ℃ for 2 min, increased at 15 ℃/min to 220 ℃, then 40 ℃/min to 270 ℃ final temperature; Injector temperature： 240 ℃ ; FID temperature： 280 ℃ ; Injector volume： 1 μL; Nitrogen was used as carrier gas, the flow of column was 1.8 mL/min. Results：The method was linear within the range from 1.0～20.0 μg/mL, the average recovery was 99.05%, and the RSD was 1.29%. Conclusions：This method is rapid, simple and accurate.

关 键 词：复方毛冬青注射液 气相色谱法 甲基正壬酮 

分 类 号：R917[医药卫生—药物分析学]