分子信标PCR检测志贺菌ipaH基因  被引量:14

Detection of Shigella Gene ipaH Using Molecular Beacons

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作  者:赵丽华[1] 周勇[1] 万成松[1] 

机构地区:[1]南方医科大学公共卫生与热带医学学院微生物学系,广州510515

出  处:《热带医学杂志》2006年第5期499-502,共4页Journal of Tropical Medicine

基  金:广东省科技计划项目(No.2002B3100101)

摘  要:目的用分子信标探针PCR快速检测志贺菌属(Shigella)。方法根据GenBank上公布的福氏志贺菌M32063株ipaH基因序列,设计引物和分子信标探针,以4种细菌进行对照,进行特异性和灵敏度分析,建立Shigella的实时PCR技术快速检测,应用于食物中毒和食品检测。结果检测20个样本,Shigella呈阳性,其它呈阴性,时间约2h。结论Shigella分子信标探针技术具有快速、灵敏度高、特异性强等特点,可用于Shigella食物中毒快速诊断和食品微生物检测,为食源性疾病的早期、准确诊断提供新的检测手段。Objective To develop a molecular beacon based fluorescence PCR assay for the detection of Shigella gene ipaH. Methods Primers and the molecular beacon were designed according to the core sequence of ipaH gene published in GenBank. Real-time PCR method was established. 20 different samples were tested. Results All Shigella strains were positively identified. Results could be obtained within two hours. Conclusion The molecular beacon-based real-time PCR assay is a rapid, sensitive, and specific method. It can be used for microbiological analysis of Shigella in foods.

关 键 词:SHIGELLA 分子信标 实时PCR ipaH 

分 类 号:R378.25[医药卫生—病原生物学]

 

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