抗牛免疫缺陷病毒（BIV）穿膜蛋白gp45杂交瘤细胞株的建立  

GENERATION OF A HYBRIDOMA REACTIVE AGAINST BOVINE IMMUNODEFICIENCY VIRUS TRANSMEMBRANE PROTEIN

作　　者：王岱[1] 魏莘[1] 刘淑红[1] 耿运琪[1] 黄建英[1] 

机构地区：[1]南开大学生命科学学院,天津市肿瘤研究所

出　　处：《南开大学学报（自然科学版）》1996年第1期59-63,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家教委优秀年轻教师基金;天津市自然科学基金

摘　　要：编码牛免疫缺陷病毒（ＢＩＶ）穿膜蛋白的一个４００ｂｐ的基因片断被克隆到ｐＡＴＨ表达载体上，此重组的融合蛋白ＴｒｐＥ－Ｅｎｖ８在大肠杆菌中得到高效表达，而且包含有与ＢＩＶ抗体特异性反应的抗原决定簇．我们用此融合蛋白免疫ｂａｌｂ／ｃ，小鼠得到与ＢＩＶ穿膜蛋白特异性反应的杂交瘤．此单克隆抗体在蛋白印迹法（ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ）分析中与重组的ＴｒＰＥ－Ｅｎｖ８有特异性反应，用免疫酶染色法可检测ＢＩＶ在体外的复制．A 400bp gene segment encoding a partial peptide of Bovine Immunodeficiency Virus (BIV)transmembrane protein was cloned into pATH expression vector. The recombinant fusion protein trpE-Env8 expressed at a high level in E. colt and containing a major immunoreactive epitope. The fusion protein was used to immunize balb/c mice to generate hybridoma specifically against BIV transmembrane protein. The Mab was specific for the TrpE-Env8 protein in western blotting assay and detected specifically the BIV replication in vitro by immunoperoxidase assay.

关 键 词：牛 免疫缺陷病毒 穿膜蛋白 单克隆抗体 杂交瘤 

分 类 号：S858.230.2[农业科学—临床兽医学]