基质衍生因子在大鼠腹主动脉移植术后介导的修复重构机制  

Repair and reconstruction mechanism of stromal cell derived factor-1 on rat abdominal aorta grafts

作　　者：高成金[1] 熊玮[2] 黄石书[3] 李幼平[3] 

机构地区：[1]复旦大学附属金山医院烧伤整形科,上海200540 [2]四川大学华西医院泌尿外科 [3]四川大学华西医院卫生部移植工程与移植免疫重点实验室

出　　处：《中华器官移植杂志》2006年第6期324-326,共3页Chinese Journal of Organ Transplantation

基　　金：国家重点基础研究-973计划资助项目(2003CB515504)

摘　　要：目的探讨基质衍生因子-1(SDF-1)在大鼠腹主动脉移植术后介导的修复重构机制。方法雄性Wistar大鼠为供者,雄性SD大鼠为受者,建立腹主动脉原位移植模型。免疫组织化学技术检测受者移植动脉内皮细胞上SDF-1的表达以及血管内膜的厚度;逆转录-聚合酶链法(RT-PCR)检测移植动脉血管中SDF-1受体CXCR4的表达。结果受者移植的腹主动脉内皮细胞上持续表达SDF-1,且其表达水平与术后血管内膜增生厚度密切相关。结论SDF-1能动员干细胞至移植的腹主动脉,并分化成平滑肌样细胞。SDF-1可能成为预警血管硬化、慢性移植物功能丧失发生和发展的指标。Objective To investigate the repair and reconstruction mechanism of stromal cell derived factor-1 （SDF-1） on rat abdominal aorta grafts. Methods Male Sprague-Darley rats received abdominal aorta grafts from male Wistar abdominal arteries. Immunohistochemical staining （IHC） was performed to detect the SDF-1 expression. The expression of CXCR4, the receptor of SDF-1, was demonstrated by RT-PCR. Results SDF-1 was expressed on the endothelium all the time during the course of the abdominal aorta grafts in the bodies of recipients. Furthermore, it was found that there was a significant relationship between the expression of SDF-1 on the endothelia and the thickness of the neointima of the rat abdominal aorta grafts. On the other hand, CXCR4 was detected within the abdominal aorta grafts. Conclusions Stem cells could be mobilized by SDF-1 to the abdominal aorta grafts and differentiated into smooth-muscle like cells. Furthermore, SDF-1 is predictive of grafts ar teriosclerosis and the subsequent development of chronic graft dysfunction （CGD）.

关 键 词：基质衍生因子-1 主动脉 腹 免疫组织化学 

分 类 号：R654.3[医药卫生—外科学]