Purification and Characterization of an Antibacterial Protein from the Cultured Mycelia of Cordyceps sinensis  

作　　者：HU Zheng YE Maoqing XIA Liqiu TU Wenjuan LI Liang ZOU Guolin 

机构地区：[1]College of Life Sciences, Wuhan University, Wuhan 430072,Hubei, China [2]Key Laboratory of Industrial Microbiology of Hubei Province,Hubei University of Technology, Wuhan 430068, Hubei, China [3]College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan, China

出　　处：《Wuhan University Journal of Natural Sciences》2006年第3期709-714,共6页武汉大学学报（自然科学英文版）

基　　金：Supported by the National Natural Science Foundation of China(39770200) ;the Natural Science Foundation of Hubei Province (2004ABA228)

摘　　要：An antibacterial protein was isolated from the cultured mycelia of Cordyceps sinensis, and was designated as Cordyceps sinensis Antibacterial Protein （CSAP）. CSAP was single-chained, with an apparent molecular mass of 35 × 10^3 revealed by SDS-PAGE and a novel hydrophobic N-terminal sequence N-ALATQHGAP. The antimicrobial assays showed CSAP could inbibit the growth of Gram-positive and Gram-negative bacteria but no significant inhibition against fungi or yeasts. Further more, the antibacterial activity of CSAP was not bactericidal but bacteriostatic. It was the first time that an antibacterial protein was described in the Cordyceps species, which might involve in the chemical defense mechanism of the hosts.An antibacterial protein was isolated from the cultured mycelia of Cordyceps sinensis, and was designated as Cordyceps sinensis Antibacterial Protein （CSAP）. CSAP was single-chained, with an apparent molecular mass of 35 × 10^3 revealed by SDS-PAGE and a novel hydrophobic N-terminal sequence N-ALATQHGAP. The antimicrobial assays showed CSAP could inbibit the growth of Gram-positive and Gram-negative bacteria but no significant inhibition against fungi or yeasts. Further more, the antibacterial activity of CSAP was not bactericidal but bacteriostatic. It was the first time that an antibacterial protein was described in the Cordyceps species, which might involve in the chemical defense mechanism of the hosts.

关 键 词：Cordyceps species antimicrobial protein ISOLATION identification membrane-activity proteins 

分 类 号：Q51[生物学—生物化学]