Expression of TGF-β_2 in LECs of Age-Related Nuclear, Cortex Cataract and the Relationship among TGF-β_2 , Proliferation, Apoptosis and Transdifferentiation  

作　　者：YE Lin CAI Xiaojun ZHANG Baifang LUO Hong Deng Ping 

机构地区：[1]Ophthalmology Department, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei, China [2]Medical School, Wuhan University, Wuhan 430071, Hubei,China

出　　处：《Wuhan University Journal of Natural Sciences》2006年第3期756-760,共5页武汉大学学报（自然科学英文版）

基　　金：Supported bythe Natural Science Foundation of Hubei Province(2000-J059)

摘　　要：To detect the pathogenesis of age-related cataract, we analyzed the expression of TGF-β2 mRNA, proliferation cell nuclear antigen （PCNA）, Bcl-2/Bax, fibronection （FN）, vimentin protein and the density of lens epithelial cells（LECs） of nuclear cataract, cortex cataract and normal LECs. Results showed that the expression level of TGF-β2 mRNA, FN and vimentin protein was higher in LECs of cortex cataract than that of nuclear cataract and normal lens. But the level of Bcl-2/Bax and PCNA was on the contrary. The density of LECs was （4250.63±275.05）/mm^2 in cortex cataract. It was （5438. 40±262.30）/mm^2 in nuclear cataract, and （5368.63 ±211.07）/mm^2 in normal LECs resepectively. There was significant difference between cortex and nuclear cataract （p〈0.05）. These suggested that TGF-β2 might take an important part in the process of age-related cataract. Age-related nuclear cataract was related to the proliferation LECs. While cortex cataract was related to the apoptosis and transdifferentiation of LECs.To detect the pathogenesis of age-related cataract, we analyzed the expression of TGF-β2 mRNA, proliferation cell nuclear antigen （PCNA）, Bcl-2/Bax, fibronection （FN）, vimentin protein and the density of lens epithelial cells（LECs） of nuclear cataract, cortex cataract and normal LECs. Results showed that the expression level of TGF-β2 mRNA, FN and vimentin protein was higher in LECs of cortex cataract than that of nuclear cataract and normal lens. But the level of Bcl-2/Bax and PCNA was on the contrary. The density of LECs was （4250.63±275.05）/mm^2 in cortex cataract. It was （5438. 40±262.30）/mm^2 in nuclear cataract, and （5368.63 ±211.07）/mm^2 in normal LECs resepectively. There was significant difference between cortex and nuclear cataract （p〈0.05）. These suggested that TGF-β2 might take an important part in the process of age-related cataract. Age-related nuclear cataract was related to the proliferation LECs. While cortex cataract was related to the apoptosis and transdifferentiation of LECs.

关 键 词：TGF-β2 PCNA Bcl-2/Bax FN vimentin CATARACT CORTEX NUCLEAR 

分 类 号：R767[医药卫生—耳鼻咽喉科]