13株新城疫病毒强毒株的分离鉴定及部分生物学特性  

Isolation and Identification of Velogenic Newcastle disease virus of 13 Strains and Their Biological Characteristics

作　　者：连宏军[1] 闫丽辉[2] 刘培欣[2] 曹殿军[2] 韩正博[2] 孙建宏[2] 梁宏志 

机构地区：[1]新疆农垦科学院新疆生产建设兵团绵羊繁育生物技术重点实验室,新疆石河子832000 [2]中国农科院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [3]哈尔滨市兽医卫生防疫站,黑龙江哈尔滨150004

出　　处：《动物医学进展》2006年第6期64-69,共6页Progress In Veterinary Medicine

基　　金：黑龙江省科学技术计划项目(GB02B505)

摘　　要：2004年-2005年从黑龙江省11个地区鸡场、鸽场、鹅场疑似新城疫(ND)病死鸡、鸽、鹅体内分离到24株具有血凝活性的病毒,经HA、HI试验和电镜观察证实了13株病毒为NDV。其鸡胚平均死亡时间(MDT)和1日龄雏鸡脑内接种致病指数(IC-PI)分别为36.8 h^73.6 h和1.51~2.00之间,属于NDV强毒株或中强毒株。除HLJ-12-04株外,强毒株的血凝素热稳定性较差,属快速或中速血凝解脱型;弱毒株的血凝素热稳定性好,属慢速血凝解脱型。所有毒株均可较好地凝集鸡和人(O型血)的红细胞,但对其他动物的红细胞凝集性差异较大,对马和兔的红细胞凝集无规律。13 strains of Newcastle disease virus （NDV）, 11 from vaccinated chickens and 2 trom pigeons, were isolated from Heilongjiang Province during 2004-2005. All isolates＇ mean death time （MDT） of embryonated eggs, interacerebral pathogenicity index （ICPI）, hemagglutination spectrum of erythrocytes, and hemagglutination-separation reactions were tested. The results showed that MDT of all NDV isolates were between 36.8 hours and 73. 6 hours,ICPI were between 1.51 and 2. 00, belonged to velogenic NDV. All 13 strains can agglutinate the erythrocytes of chicken and human O type. However, their ability of agglutinating erythrocytes of swine, horse, cow, rabbit and guinea pig were variable. Accept from HLJ-12-04, the hemagglutinin of lentogenic strains were stable to heat and belonged to slow type of agglutination-separation, virulent strains were not stable to heat and belonged to fast type of agglutination-separation.

关 键 词：新城疫病毒 分离鉴定 生物学特性 毒株 

分 类 号：S852.659.5[农业科学—基础兽医学]