HPLC法测定人血浆中右旋酮洛芬的浓度  

Determination the Content of Dexketoprofen in Human Plasma by HPLC

作　　者：管潇[1] 张梦军[1] 邬晓薇[1] 张惠静[1] 

机构地区：[1]第三军医大学医学检验系分析化学教研室,重庆400038

出　　处：《中国药师》2006年第6期540-541,共2页China Pharmacist

摘　　要：目的:建立HPLC法测定人血浆中右旋酮洛芬的浓度。方法:血浆样品以乙醚为萃取剂,萘普生为内标物;采用Shim-pack VP-ODS色谱柱(150 mm×4．6 mm,5μm);流动相为乙腈-5 mmol·L-1磷酸缓冲液(22:78,pH=6．8),流速1．0 ml·min-1;紫外检测波长262 nm。结果:右旋酮洛芬血浆样品的线性范围为0．02-20．00μg·ml-1,r=0．999 9,n=8。萃取回收率和方法回收率分别为72．28％-75．63％和104．0％-107．8％(n=5),日内、日间RSD分别为0．6％-6．2％和2．7％-4．8％(n=5)。结论:本法简便,准确,重现性好。Objective： To establish a high performance liquid chromatographic （HPLC） method for the separation and determination the content of dexketoprefen in human plasma. Method： Shim-pack VP-ODS column（ 150 mm ×4.6 mm, 5μm） was used, acetonitrile-5 mmol. L^-1 phosphas buffer （22： 78, v/v, pH = 6.8） as mobile phase pumped at 1.0 ml· min^-1 and quantified by ultraviolet detection with wavelength set at 262 nm. Result： The range of calibration curve was 0.02-20.00 μg. ml^-1 and the correlation coefficient was 0.9999. The extraction recovery was 72.28%-75.63% and the method recovery was 104.0%-107.8%. The relative standard deviations for within-day, between-day were 0.6%～6.2%, 2.7% -4.8% respectively. Conclusion： It is a simple, accurate and reproducible method which can be applied successfully to the study of pharmacokinetics and bioavaila bility of dexketoprefen.

关 键 词：右旋酮洛芬 高效液相色谱法 血浆 药物浓度 

分 类 号：R927.2[医药卫生—药学]