Frankia共生固氮基因的分离与克隆  

Isolation and Cloning of Symbiotic Nitrogen-fixing Genes in Frankia

作　　者：陈丽梅[1] 柏学亮[1] 彭源东[1] 

机构地区：[1]广西农业大学实验中心分子遗传学研究室,沈阳应用生态研究所

出　　处：《广西农业大学学报》1996年第1期46-49,共4页

基　　金：国家攀登计划资助

摘　　要：利用ＤＮＡ－ＤＮＡ杂交方法，分离细枝木麻黄的Ｆｒａｎｋｉａ菌株Ｃｏ０１的ｎｉｆ克隆ｐＣｃ１ＧＸ，赤杨内生菌株Ａｔ４的ｎｉｆ克隆ｐＡｔ１ＧＸ及沙棘的ＦｒａｎｋｉａＨｒ１８的ｎｉｆ克隆ｐＨｒ１８ＧＸ、ｐＨｒ１１－③ＧＸ。用基因功能互补法，从Ｆｒａｎｋｉａ菌株Ａｔ４的基因文库中分离到二个可能可互补豌豆根瘤菌ｎｏｄ基因功能的克隆ｐＡｔ２ＧＸ、ｐＡｔ３ＧＸ，并制作了ｐＡｔ２ＧＸ、ｐＡｔ３ＧＸ的亚克隆，予进一步实验，获得充分的证据证明ｐＡｔ２ＧＸ、ｐＡｔ３ＧＸ是否带有Ｆｒａｎｋｉａ菌株Ａｔ４的ｎｏｄ基因．Using DNA-DNA hybridizations, nif clones of Frankia pCclGX from Co01,pAt1GX from At4, pHr18GX and pHr11-③ GX from Hr18 were isolated. By complementation with Rhizobium leguminosarum mutant nodD:: Tn5, two clones pAt2GX and pAt3GX were isolated from the Frankia strain At4 bank. The two clones were subcloned and further experimented to confirm whether they contain nodD gene of Frankia At4.

关 键 词：共生固氮 结瘤 基因 功能互补 

分 类 号：S154.383[农业科学—土壤学]