胆碱乙酰基转移酶活性快速测定诊断臂丛损伤的实验研究  

Experimental study on diagnosing the brachial plexus injury through quickly detecting the ChAT activity

作　　者：李智富[1] 许扬滨[1] 刘长征[2] 

机构地区：[1]中山大学附属第一医院整形外科,广州市510080 [2]中山大学中山医学院核医学教研室

出　　处：《中华显微外科杂志》2006年第3期194-197,共4页Chinese Journal of Microsurgery

摘　　要：目的寻找一种简单快速、能在手术中准确客观地判断臂丛损伤神经残端功能的方法。方法选用成年雌性SD大鼠45只,随机分成两组,分别制成臂丛节前和节后损伤的动物模型,采用放射性同位素的方法测定损伤后不同时间臂丛神经中胆碱乙酰基转移酶(choline acetyltransferase,ChAT)的活性,然后用统计学的方法总结其中规律。结果节前、节后两种损伤类型胆碱乙酰基转移酶的活性均随时间降低,节前组ChAT活性随时间迅速降低,很快达到正常活性的1/10(675±258) cpm；节后组ChAT活性随时间缓慢降低,90 d后只降低到正常活性的1/2(4906±1119)cpm。结论术中快速测定ChAT的活性可以用来定量地区分臂丛损伤后的神经残端功能,以判定残端的可利用性。Objective To explore a better method to diagnose the brachial plexus injury as pre-or post-ganglionic, which is quick, simple, quantitative, cheap and accurate. Methods Forty-five SD rats were divided randomly ino pre-or post-ganglionic brachial plexus injury models group, and the choline acetyltransferase（CHAT） activity was detected by using the radioisotope after different days. Results The ChAT activity of two groups both fall day by day： in the pre-agnglionic group, it falls quickly, and reach 1/10 of normal（675±258 ）cpm; in the post-ganglionic group, it falls slowly, and reach 1/2 of normal even after 90 days （4906±1119 ） cpm. Conclusion Intra-operative measure of ChAT can be used to differentiate pre-or post-ganglionic brachial plexus injury.

关 键 词：胆碱乙酰基转移酶 放射性同位素 臂丛损伤 神经残端功能 

分 类 号：R651.3[医药卫生—外科学]