间接ELISA法对H pylori免疫牛初乳中抗体的检测  

Indirect ELISA in detection of antibodies in colostrums of the cows immunized with Helicobacter pylori

作　　者：周冬仁[1] 王锋[1] 张常印 崔山[1] 唐泰山 偶健[1] 

机构地区：[1]南京农业大学动物科技学院,江苏省南京市210095 [2]江苏省进出境检验检疫局动检实验室,江苏省南京市210001

出　　处：《世界华人消化杂志》2006年第11期1103-1106,共4页World Chinese Journal of Digestology

基　　金：江苏省国际合作项目;No.BZ2005041

摘　　要：目的:建立间接ELISA法测定牛初乳中H pylori抗体的水平,并观察特异性抗体的产生规律．方法:本试验以H pylori全菌抗原免疫妊娠母牛,每头牛每次肌肉注射4×109CFU全菌抗原, 收集分娩后7 d内的初乳．以间接ELISA法检测其中H pylori抗体水平．结果:H pylori能有效地引起免疫应答,成年牛初乳中的抗体效价在分娩当天达到最大值 5．84,高于青年牛的3．51(P<0．05),随后迅速下降．对照奶牛仅在分娩当天检测到微弱阳性．结论:成年牛的对H pylori的免疫应答好于青年牛．AIM： To observe the changes of specific antibodies in the cow colostrums by indirect enzyme linked immunosorbent assay （ELISA）. METHODS： Two pregnant cows （non-parous and parous） were immunized with H pylori whole-cell antigen （4×10^9 CFU） via intramuscular injection in the neck. The colostrums were collected with 7 days after parturition. RESULTS： H pylori induced significant immune responses in both cows, and the efficacy of anti-H pylori in the parous cow reached the peak values （5.84） at the first day after parturition, which was significantly higher than that in the non-parous cows （3.51, P 〈 0.05）. However, the efficacies dropped quickly with 7 days. Weak positive anti-H pylori were observed only at the first day in the control cow. CONCLUSION： The immune response is superior in the parous cow to that in the non-parous one.

关 键 词：间接ELISA 幽门螺杆菌 初乳 

分 类 号：S852.4[农业科学—基础兽医学]