丹参对烧伤大鼠肝细胞线粒体呼吸功能的影响  

Effect of salvia miltiorrhiza on mitochondria respiratory function of hepatic cells in rats with burn

作　　者：邱振中[1] 李锐[1] 邱财荣[1] 马黎军[1] 顾磊奇 唐晋[1] 

机构地区：[1]解放军第三医院,陕西宝鸡721004

出　　处：《中国中西医结合急救杂志》2006年第3期156-158,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：陕西军区医药卫生科研计划项目(LXH2005003)

摘　　要：目的:探讨丹参对烧伤大鼠肝细胞线粒体呼吸功能及氧自由基的影响。方法:将96只SD大鼠按随机数字表法分为正常对照组(A)、模型组(B)和丹参组(C);B组和C组造成20%总体表面积Ⅲ度烫伤。在伤后1、2和6 h动态检测肝细胞线粒体内细胞色素aa3、细胞色素C、能荷和超氧化物歧化酶(SOD)水平。结果:①伤后2 h内,B、C组肝细胞线粒体内细胞色素aa3水平均无显著降低(P均>0.05)。伤后6 h,B、C组肝细胞线粒体内细胞色素aa3水平显著低于A组(P均<0.05);同时C组显著高于B组(P<0.05)。②伤后1 h开始,肝细胞线粒体内细胞色素C水平、能荷和SOD活性下降;且C组显著高于B组(P均<0.05)。结论:丹参能显著提高肝细胞线粒体内细胞色素aa3、细胞色素C、能荷和SOD水平;明显改善大鼠烧伤后肝细胞线粒体呼吸功能,减少氧自由基的产生。Objective： To study the effect of salvia miltiorrhiza on mitochondria respiratory function of hepatic cells and oxygen radicals in rats with burn. Methods： Ninety-six Sprague-Dawley rats were randomly divided into normal control group, model group and salvia miltiorrhiza group. Rats in model group and salvia miltiorrhiza group were subjected to a 20% total body surface area （TBSA） full thickness thermal injury. The levels of cytochrome aa3, cytochrome C, energy production and superoxide dismutase （SOD） in mitoehondria of hepatic cells were dynamically determined at 1, 2 and 6 hours after injury. Results： ①The levels of cytochrome aa3 in model group and salvia miltiorrhiza group did not change obviously within 2 hours after burn, while they were much lower than that in normal control group at 6 hours after burn, and the level of cytochrome aa3 in salvia miltiorrhiza group was obviously higher than that in model group （all P〈0. 05）. ②From 1 hour after burn, the levels of cytochrome C, energy production and SOD in model group and salvia miltiorrhiza group decreased, and the levels was higher in salvia miltiorrhiza group obviously than those in model group （all P〈0.05）. Conclusion： Salvia miltiorrhiza could enhance the levels of cytochrome aa3, cytochrome C, energy production and SOD, which could obviously protect mitochondria respiratory function of hepatic cells and obviously reduce the production of oxygen free radicals.

关 键 词：烧伤 丹参 肝脏 线粒体 氧自由基 大鼠 

分 类 号：R285[医药卫生—中药学]