用于输血四项指标检测的压电免疫传感器阵列的研制  

Research on the Piezoelectric Immunosensor Array Used for Detection of the Four Blood Index

作　　者：李艳霞[1] 刘仲明[1] 张水华[2] 刘毅新[1] 黄胜宾 徐蓓蓓 

机构地区：[1]华南理工大学食品与生物工程学院,广东广州510642 [2]广州军区广州总医院医学实验科,广东广州510010 [3]佛山分析仪器有限公司,广东佛山528041

出　　处：《中国医学物理学杂志》2006年第3期185-187,223,共4页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(69977Ω21);全军医学科研"十五"计划基金课题重点项目(02Z014)

摘　　要：采用聚乙烯亚胺粘附,戊二醛交联,固定相应的敏感材料研制了四通道压电免疫传感器阵列。该阵列可快速同步检测乙肝表面抗原(HBsAg)、丙肝病毒抗体(HCV)、梅毒螺旋体抗体(TP)、爱滋病毒(HIV1+2)抗体,考察了该传感器阵列的灵敏度、特异性、稳定性,并应用于临床样品的检测,同时与ELISA法进行比较。结果表明阳性血清的响应值与阴性血清的噪声值之比均大于4,可用于输血四项指标的同步检测,其检测灵敏度略高于ELISA法。The corresponding sensitive material was fixed on the four-channel piezoelectric immunosensor array by polyethyleneimine adhesion and glutaraldehyde cross-linking method. This array could detect hepatitis B surface antigen, anti-hepatitis C virus,anti-TP,anti-HIV1＋2. Its sensitivity,specificity and stability were detected and the human serum samples had been also detected,besides,it compared with the ELISA. Results showed that respond of the positive serum was 4 times higher than negative serum and its was better than ELISA a little sensitivity.

关 键 词：乙肝表面抗原(HBsAg) 丙肝病毒抗体(HCV) 梅毒螺旋体抗体(TP) 爱滋病毒(HIV1+2)抗体 石英晶体 阵列 

分 类 号：R446.111[医药卫生—诊断学]