红霉素抑制人工关节磨屑诱导的骨溶解  

Erythromycin inhibits wear debris-induced osteolysis

作　　者：高绪仁[1] 范卫民[1] 李翔[1] 马益民[1] 崔维顶[1] 

机构地区：[1]南京医科大学第一附属医院骨科,210029

出　　处：《江苏医药》2006年第6期514-515,共2页Jiangsu Medical Journal

摘　　要：目的探讨红霉素(EM)防治人工关节松动的可能性。方法采集30例人体外周血,分离单核细胞(MO)。每例标本分成6组。A组:仅MO;B组:MO+超高分子聚乙烯微粒(UHMWPE);C组:MO+UHMWPE+帕米磷酸钠(10μg/ml);D组:MO+UHMWPE+EM(5μg/ml);E组:MO+UHMWPE+EM(10μg/ml);F组:MO+UHMWPE+EM(25μg/ml)。培养48 h后,放免法测定上清中肿瘤坏死因子(TNF)含量。结果A、C、D、E、F组的TNF含量明显低于B组。结论EM能有效地抑制由于UHMWPE刺激MO而分泌的TNF的量。EM的效果与帕米磷酸钠相似,能防治人工关节置换术后由微粒诱导的炎症性因子引发的骨溶解,有望成为将来防治人工关节无菌性松动的一种很有潜力的药物。Objective To explore the possibility of erythromycin（EM） in preventing and treating aseptic loosening of prothesis. Methods Peripheral blood samples were obtained from healthy human donors. Monocytes（MOs） were isolated and assigned to 6 groups. Group A was treated with MO alone, group B with MO＋ ultrahigh molecular weight polylethylene（UHMWPE） debris, group C with MO＋UHMWPE＋pamidronate（ 10 μg/ml）, group D with MO＋ UHMWPE＋ EM（5 μg/ml）, group E with MO-FUHMWPE-FME（10 μg/ml） ,and group F with monocytes-FUHMWPE-FEM（25 μg/ml）. The cells were co-cultured for 48 hours and the TNF was determined. Results The levels of TNF were much higher in group B than those in group A, C, D, E and F（P〈0. 01）., which were much lower in group C than those in group B（P〈0.01） ,and much higher in group D than those in group F （P〈0. 05）. Conclusion EM inhibits effectively UHMWPE-stimulated TNF secretion of MOs and appears to be a potential therapeutic candidate for the treatment and prevention of aseptic loosening.

关 键 词：红霉素 二磷酸盐 磨屑 假体失败 骨溶解 

分 类 号：R687.4[医药卫生—骨科学] R285.5[医药卫生—外科学]