肝移植后供肝和宿主免疫学状态的变化  

Change of the immmunologic state of liver graft and host after liver transplantation

作　　者：古维立[1] 翁杰锋[1] 夏金堂[1] 山口淳三 兼松隆之 

机构地区：[1]广州市第一人民医院肝胆外科,510180 [2]日本长崎大学医学部第二外科

出　　处：《中华实验外科杂志》2006年第6期673-674,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察供肝免疫原性和宿主对供体抗原反应能力的动态改变。方法利用近交系LEW到WF的大鼠肝移植自发免疫耐受模型,取出不同时期的供肝,分别刺激长期生存的WF宿主,观察能否诱导出肝损害；另外对LEW→WF肝移植后不同时期的宿主,再次给予供体抗原刺激。结果(1)移植后第1、2天的同种移植肝,可激起长期生存的宿主出现暂时性肝损害(121±33、83±21),但第3天以后的则不能(28±9)。(2)给予供体同源的脾细胞刺激后,移植后7、14、28 d的宿主均未能诱导肝损害(56±17、66±11、61±35),但第56、84或112天的宿主均可被诱导出暂时性肝损害(98±25、158±43、330±82)。结论(1)肝移植后供肝的免疫原性在术后3 d基本消失。(2)移植术后1个月内宿主对供体抗原的刺激是处于低(无)反应状态的。Objective To study the immunogenicity of the liver graft and the dynamic change ot the reaction ability from host againstdonor antigen. Methods Donor livers at different stages were taken out from the Lewis（LEW） to Wistar（WF） rat model of liver transplantation. Long-term survial WF host was stimulated by the liver tissues. The host at different stages after LEW to WF transplantation was treated by donor antigenic stimulation. Hepatic lesion of the host was estimated. Results （ 1）The homograft tissues, only at the 1st or 2nd day not the 3rd dayor more after transplantion, caused temporal hepatic lesion of the long-term survival host; （2）For the hosts at 7th, 14th or 28th day after transplantation, hepatic lesion was not induced by the spleen cells stimulation. But temporal hepatic lesion appeared in the hosts at 56th, 84th or 112th day after transplantation. Conclusion （ 1 ） Graft immunogenicity disappears at the 3rd day after liver transplantation. （2）The host in less than 30 days after transplantation is of hyporesponsiveneas to the donor antigen.

关 键 词：肝移植 抗原呈细胞 免疫学 

分 类 号：R657.3[医药卫生—外科学]