SSR和STS标记在花生栽培品种鉴定中的应用研究  

SSR and STS Polymorphisms in Seed Testing for Peanut Cultivars

作　　者：张建成[1] 王传堂[1] 杨新道[1] 

机构地区：[1]山东省花生研究所,青岛266100

出　　处：《植物遗传资源学报》2006年第2期215-219,共5页Journal of Plant Genetic Resources

基　　金：国家"973"前期专项(2002CC03200);国家自然科学基金项目(30300224)

摘　　要：采用10份花生品种材料,对通过数据库查询设计合成的SSR引物和其他作者发表的SSR引物及STS引物进行了评估,并基于品种间简单匹配系数做了聚类分析。获得62个能产生多态性片段的SSR和STS引物对,总共获得427条带,其中291条(68.1%)为多态性带。平均每对引物产生6.88条带,4.69条为多态性带;多态性条带比率为16.7%～100%,PIC值为0.254～0.952,平均为0.760。9对SSR/STS引物,即Lec1、Ah426、Ah44、SsS14、SHPAL1、PG71、PG43、PM36、PG22,对所采用的10份花生品种区分率达到100%。说明SSR和STS标记应用于花生品种鉴定有效。SSR/STS primers published for peanut and related taxa, along with those from Genebank search were evaluated using 10 peanut cultivars mainly from Shandong. Cluster analysis was carried out based on simple matching coefficients between varieties. Sixty-two SSR/STS primer pairs were found to produce 427 polymorphic bands, and 291（68.1%） were polymorphic. On an average, each primer pair produced 4.69 polymorphic bands in a total of 6.88 bands. The polymorphic percentage and PIC ranged from 16.7 % - 100 % and 0.254 - 0.952, respectively. Nine primer pairs, viz, Lec-1、Ah4-26、Ah4-4、SsS14、SHPAL-1、PG71、PG43、PM36、PG22, may differentiate each of the individual peanut cultivars. The present study demonstrated the practicability of SSR/STS markers in the varieties identification of peanut. The high genetic similarity of Shandong peanut at DNA level indicated narrow gene bases, which need broadening in cultivar breeding through utilization of elite exotic germplasm resources.

关 键 词：花生 栽培种 微卫星 序列标签位点 多态性 

分 类 号：S565.2[农业科学—作物学]