新生儿感染性疾病中性粒细胞CD64分子变化及临床意义  

The expression of peripheral blood neutrophil CD64 in neonatal infection.

作　　者：赵方[1] 王琳[1] 聂李平[2] 彭华[2] 刘瑞[2] 曾宾[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院儿科 [2]北京大学深圳医院检验科儿科,深圳518036

出　　处：《中国优生与遗传杂志》2006年第6期31-32,38,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的观察新生儿感染性疾病中性粒细胞CD64表达水平及其临床意义。方法将34例新生儿感染性疾病患儿根据其临床表现及实验室检查结果分为败血症12例,非败血症感染22例,以同期收治的非感染性新生儿28例为对照组。采用全血流式细胞术、放射酶联免疫吸附试验及特定蛋白分析仪比浊法分别检测三组患儿CD64指数、IL-6、CRP水平。结果败血症组CD64指数、IL-6及CRP水平均高于非败血症感染组和对照组,差异有显著性意义(P<0.05),非败血症感染组CD64指数、IL-6及CRP水平高于对照组(P<0.05);感染组CD64指数与IL-6呈显著的正相关(P<0.01);败血症组CD64指数随病情好转而逐渐降低。结论新生儿周血中性粒细胞CD64表达水平在感染性疾病时显著升高,并随病情而变化,早期检测意义较大。Objective ： Neonatal infection is common disease. This study was to investigate expression of neutrophil adhesion molecule CD64 in bacterial infection and its significance. Methods： According to the diagnostic criteria for neonatal septicemia, the patients were divided into septicemia group （ n = 12） and non - septicemic infection group （ n = 22 ）. Twenty - eight hospitalized neo- nates with non - infection disease were enrolled as controls. The levels of peripheral blood neutrophil CD64 were measured by using flow cytometry. We also assessed the level of serum CRP and IL - 6. Results ： The expression level of neutrophil CD64 and the levels of IL - 6, CRP in the septicemia group were significantly higher than those in the control group and non - septicemic infection group （ P 〈 0. 05 ）. The expression level of neutrophil CD64 and the levels of IL - 6, CRP in non - septicemic infection group were also sig- nificantly higher than those in the control group （P 〈 0. 05 ） .. The expression of neutrophil CD64 showed a positive correlation to the levels of IL - 6 （P 〈 0. 01 ） ; The expression of neutrophil CD64 changed with the condition of the sick neonates. Conclusion： The expression of neutrophil CD64 was increased in the bacterial infection and changed with the condition of the sick neonates. Therefore, it could be useful for diagnostic and prognostic marker for neonate bacterial infection.

关 键 词：感染性疾病 新生儿 流式细胞术 中性粒细胞 

分 类 号：R722.13[医药卫生—儿科]