小鼠脾细胞CD4^+ CD25^+调节性T细胞的表型特征  

Phenotype of mouse CD4^+ CD25^+ regulatory T cells in mouse splenocytes

作　　者：杨滨燕[1] 沈二霞[1] 吴长有[1] 

机构地区：[1]中山大学基础医学院免疫学教研室,广州510080

出　　处：《现代免疫学》2006年第3期208-212,共5页Current Immunology

基　　金：国家重点基础研究发展计划项目(2001CB510007);国家自然科学基金创新群体资助项目(30321004)

摘　　要：观察CD4+CD25+T和CD4+CD25-T细胞的表型和细胞因子的表达。自小鼠脾脏制备单个细胞悬液,分离CD4+T细胞、CD4+CD25+和CD4+CD25-T细胞,进行细胞表面标记,激活后进行细胞内细胞因子染色,利用流式细胞仪在单个细胞水平上分析细胞表面分子、转录因子和细胞因子表达之间的关系。结果:在CD4+T细胞中,约有7.8%的细胞同时表达CD25分子。与CD4+CD25-T细胞相比,CD4+CD25+T细胞CD44的表达略有增加,CD45RB的表达明显下降,CTLA-4和Foxp3明显增加。以同时表达CTLA-4和Foxp3的细胞为主,其次为单独表达Foxp3的细胞。细胞因子的研究结果表明,与CD4+CD25-T细胞相比,CD4+CD25+T细胞IL-2、IFN-γ明显减少,而只产生IL-10的细胞略有增加。CD4+CD25+调节性T细胞无论在表型、转录因子的表达以及细胞因子表达方面均于非调节性T细胞不同。To compare the phenotype and cytokines expression in CCD4^＋CD25^＋T and CD4^＋CD25^-T cells, single cell suspensions from mouse spleen were prepared and the CD4^＋ . CD4^＋CD25^＋、 CD4^＋CD25^-T cells were isolated. The expression of surface molecules, transcriptional factors and eytokines was determined and analysed at the single cell level by FACS. It was found that 7.8% of CD4^＋T cells expressed CD25. Compared with CD4^＋CD25^-T cells, expression of CD44 on CD4^＋CD25^＋T cells increased slightly, CD45RB decreased remarkably, CTLA-4 and Foxp3 increased significantly. The majority of CD4^＋CD25^＋T cells expressed both CTLA-4 and Foxp3, and much less cells expressed Foxp3 alone. The results of intraeellular eytokines staining indicated that the frequency of IL-2^＋ and IFN γ^＋ CD4^＋CD25^＋T cells decreased remarkably, and the frequency of IL-10^＋ CD4^＋CD25^＋T cells increased lightly compared with CD4^＋CD25^-T cells. It is evident that the CD4^＋CD25^＋ regulatory T cells differs both in the phenotype and the expressions of transcriptional factors and eytokines, with the CD4^＋CD25^- non-regulatory T cells cells. It was different in phenotype, expression of transcriptional factors and cytokines in CD4^＋CD25^＋ T cells and CD4^＋CD25^-T cells.

关 键 词：CD4^+CD25^+T细胞 调节性T细胞 细胞表型 Foxp3 细胞因子 

分 类 号：R392.12[医药卫生—免疫学]