药用植物党参的RAPD分析  

Analysis of RAPD on Medicinal Plants of Codonopsis pilosula

作　　者：张建清[1] 苏雪[1] 吴琼[1] 丁松爽[1] 孙坤[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《中药材》2006年第5期417-420,共4页Journal of Chinese Medicinal Materials

基　　金：甘肃省教育厅科研项目;西北师范大学重点学科经费资助

摘　　要：目的:从DNA分子水平上分析甘肃党参主要栽培区的9个栽培居群和4个自然居群的遗传多样性和遗传关系,进而探讨纹党参的来源。方法:从200条随机引物中筛选出14个引物,对217个体进行RAPD扩增并计算多态性条带,再用NTSYS软件聚类分析。结果:14个引物共检测出125个可重复的位点,其中党参及其变种素花党参的多态位点分别为109和106,多态位点百分率为87．20％和84．80％。13个居群聚为两大类:8个党参栽培居群为一类,素花党参包括1个文县栽培居群与4个自然居群为第二类。结论:RAPD分析结果揭示了甘肃栽培的党参和素花党参在居群水平上具有丰富的遗传多样性。8个栽培党参居群间的遗传分化很小,与地理距离存在一定的相关性。在甘肃各地的栽培党参中,仅文县栽培品为素花党参,即正品纹党。Objective ： To study genetic diversity and genetic relationship among 9 cultivated and 4 nature populations of Codonopsis pilosula and C. pilosula var. modesta form Gansu Province at DNA level. Methods： DNA of 217 individuals was amplified by 14 decamer primers from 200 radom primers. The polymorphic bands of RAPD were counted, And the results were analyzed by clustering analysis NTSYS-PC software. Results： A total of 125 loci were detected by 14 arbitrary primers, among which 109 and 106 loci were polymorphic in C. pilosula and C. pilosula var. modesta respectively, and the percentage of polymorphic bands （PPB） were 87. 20% and 84. 80%. The cluster analysis showed that 13 populations were divided into 2 groups. The one included 8 cultivated populations of C. pilosula, the other group consisted of a cultivated populatiobn and 4 nature populations of C. pilosula var. modesta. Conclusion： The RAPD results indicated that there was abundant genetic diversity in cultivated populations of C. pilosula and C. pilosula vat. modesta. The genetic differentiation among 8 cultivated populations of C. pilosula was very small and related to their geographical distance. Only the cultivation in Wen County originated from C. pilosula vat. modesta, while the other populations cultivated in Gansu Province belongs to C. pilosula.

关 键 词：药用植物 党参 RAPD 遗传多样性 

分 类 号：R282.4[医药卫生—中药学]