胃康宁含药血清对胃癌MGC-803细胞增殖的抑制作用及机理研究  

Anti-proliferation Effect of Serum Containning WEI KANG NING on Gastric Cancer MGC-803 Cells and Its Mechanism

作　　者：冯春霞[1] 李庆明[2] 梁蔚文[2] 魏菁[2] 

机构地区：[1]中山大学第五附属医院,广东珠海519000 [2]中山大学第二附属医院,广东广州510120

出　　处：《中药材》2006年第5期456-458,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:观察胃康宁含药血清对胃癌细胞的增殖影响,从细胞凋亡角度探讨作用机理。方法:制备胃康宁含药血清培养胃癌细胞,采用MTT法检测细胞增殖,Hoechst DNA荧光染色法检测细胞凋亡,并计数凋亡率。结果:胃康宁各剂量组在初期均有抑制细胞增殖的作用,而高、中剂量抑制时间更持久。胃康宁高剂量组具有诱导细胞凋亡的作用。结论:胃康宁含药血清可明显抑制细胞增殖,其机理除与诱导细胞凋亡有关,还与细胞周期受阻也有关系。Objective ： To investigate the anti-proliferation effect of WEI KANG NING on gastric cancer. Methods ： MGC-803 cells were cultured in different concentrations of serum containning WEI KANG NING. The inhibitory ratio of the ceils was measured by MTT assay. Apoptosis was observed by Hoechst 33258 fluorescence staining. Results ： Each concentration of serum containning WEI KANG NING could inhibit cells proliferation. Among them, both high and medium concentration could inhibit longer. Moreover, high concentration could induce cells apoptosis. Conclusion： WEI KANG NING serum has anti-proliferation effect on MGC-803 cells and the effect may be related to cell apoptosis induced by WEI KANG NING.

关 键 词：胃康宁 细胞增殖 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]