安宫牛黄散中朱砂、雄黄对外伤性脑水肿大鼠热休克蛋白、一氧化氮合酶和炎症细胞因子的影响  

Effects of Cinnabar and Realgar in Angong Niuhuang Powder on Heat Shock Protein, Nitric Oxide Synthase and Inflammatory Cytokines in Contusion Cerebral Edema

作　　者：林璞粤[1] 汤毅珊[1] 王宁生[1] 

机构地区：[1]广州中医药大学临床药理研究所,广东广州510405

出　　处：《中药材》2006年第5期458-461,共4页Journal of Chinese Medicinal Materials

基　　金：广东省自然科学基金项目(010270)

摘　　要：目的:探讨安宫牛黄散中朱砂、雄黄的药理作用机制。方法:SD大鼠随机分成6组(12只／组):正常对照组、外伤性脑水肿膜型对照组、朱砂组(0．15 g/kg)、雄黄组(0．15 g/kg)、安宫牛黄散(下简称整方)组(1．5 g/ kg)、去朱砂、雄黄的安宫牛黄散(下简称拆方)组(1．2 g/kg),以上各组再设8 h分组和24 h分组(6只／分组)。RT-PCR法测定脑组织中热休克蛋白70(HSP70)mRNA表达;比色法测定脑组织中一氧化氮合酶(NOS)及其同工酶(iNOS、cNOS)活力;放免法测定血清中细胞因子TNF-α、IL-1β水平。结果:造模后8 h,整方组与拆方组HSP 70 mRNA表达均较模型组增高(P<0．05),且整方组诱导HSP 70 mRNA表达的作用强于拆方组(P<0．05);朱砂、雄黄、整方和拆方均能降低iNOS活力,且较模型组明显降低(P<0．05),其中整方组抑制作用最为显著。雄黄组、整方组、拆方组TNF-α水平较模型组降低(P<0．05),整方组与拆方组IL-1β水平较模型组降低(P<0．05)。结论: HSP 70、iNOS、TNF-α及IL-β参与了外伤性脑水肿的病理过程。安宫牛黄散和复方中的朱砂、雄黄对外伤性脑水肿大鼠脑组织的保护作用可能与通过增加诱导HSP 70 mRNA表达、抑制iNOS活力和炎症细胞因子TNF-α、IL-1β的生成有关。Objective： To explore the pharmacological mechanism of Cinnabar and Realgar in Angong Niuhuang powder（ANP）. Methods： SD rats were randomly divided into six groups（ 12 rats/group） ： normal controls group（ NS group）, contusion cerebral edema model group（ CCE group） , cerebral edema rats administrated by cinnabar 0. 15 g/kg 1h（ CA group） , cerebral edema rats administrated by realgar 0. 15 g/kg 1h（ RG group） , cerebral edema rats administrated by Angong Niuhuang powder 1.5 g/kg 1h（ANP Ⅰ group） , cerebral edema rats administrated by Angong Niuhuang powder substracted cinnabar and realgar 1.2 g/kg 1h（ ANP Ⅱ group）. Each group was divided into two subgroups（6 rats/subgroup）. The rats in subgroups were killed at 8h and 24h after modeling respectively. Expression of heat shock protein 70 （ HSP 70 ） mRNA in brain tissues was measured by RT-PCR. Activities of nitric oxide synthase （NOS） and its isoenzymes（ iNOS, eNOS）in brain tissues were tested by eolorimetry. Levels of tumor necrosis factor-α（TNF-α） and inteleukin-1β（ 1L-1β） in serum were determined by radioimmunoassay （RIA）. Resuhs ： Expression of HSP 70 mRNA in ANP Ⅰ group and ANP Ⅱ group significantly increased as compared with CCE group 8h after being modeled（ P 〈0. 05 ）, and increase range in ANP Ⅰ group were singificantly higher than that in ANP Ⅱ group（P 〈0. 05）. Activities of iNOS in CA group, RG group, ANP Ⅰ group and ANP Ⅱ group were lower than that in CCE group 8h after being modeled（ P 〈0. 05）, and the activities in ANP Ⅰ group were the lowest in the four groups. Levels of TNF-α in RG group, ANP Ⅰ group and ANP Ⅱ group decreased obviously as compared with CCE group 8h after being modeled（P 〈 0. 05）, so did levels of IL-1β in ANP Ⅰ group and ANP Ⅱ group（ P 〈0. 05）. But no significant difference was shown between ANP I group and ANP Ⅱ group. Conclusion ： HSP 70, iNOS, TNF-α and IL-1β are involved in contusion cerebral edema. AN

关 键 词：安宫牛黄散 朱砂 雄黄 热休克蛋白 一氧化氮合酶 肿瘤坏死因子-α 白介素-1β 外伤性脑水肿 

分 类 号：R285.5[医药卫生—中药学]