非致炎浓度的福尔马林激活小鼠皮肤辣椒素受体  

THE ACTIVATION OF CUTANEOUS VR1 IN MICE BY FORMALIN OF NON-INFLAMMATORY DOSE

作　　者：严彦[1] 李淑方[1] 乔永康[1] 王培[1] 曹毅[1] 周砚青[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《中国疼痛医学杂志》2006年第2期94-97,共4页Chinese Journal of Pain Medicine

基　　金：国家科技部"十五"科技攻关计划基金(2001BA704B01;2004BA809B0604;2004BA809B0605)

摘　　要：目的:探索非致炎浓度的福尔马林对小鼠皮肤辣椒素受体(vanilloid receptor type 1,VR1)的激活作用。方法:选用KM小鼠随机分为3组:福尔马林(formalin,FM)组、辣椒素(capsaicin,CAP)组和Capsazepine(CPZ)组。采用注射后5 m in内小鼠舔舐注射脚掌的时间作为评价疼痛反应的指标,对各组小鼠的痛反应进行观测。结果:0.025%FM和200μmol/L CAP是理想的实验浓度,既能引起显著疼痛反应,又不引起炎性损伤的致痛浓度,适于研究受体拮抗剂的抗疼痛作用;1μmol/L CPZ分别与200μmol/L CAP和0.025%FM共注射能有效的拮抗CAP和FM引起的疼痛反应。结论:非致炎浓度FM所致的疼痛反应在外周可能由VR1介导。Objective ： To explore the effects of cutaneous formalin （FM） injection of non-inflammatory dose on the activation of vanilloid receptor type 1 （ VR1 ） in mice. Methods ： KM mice were randomly divided into 3 groups： FM group, capsaicin （CAP） group and capsazepine（CPZ） group. The licking time of the injected paws within 5 min after injection was recorded and considered as an index of pain. Resuits： 0. 025% FM and 200μmol/L CAP were optimal concentrations of the pain reagent, which could evoke painful sensation without inflammation. 1μmol/L CPZ had obvious anti-nociceptive effects on the FM- and CAP- induced pain. Results： Pain induced by non-inflammatory dose of FM in peripheral was possibly mediated by VR1.

关 键 词：福尔马林 辣椒素受体 外周痛觉传导 

分 类 号：R402[医药卫生—临床医学]